This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lodes, M. J.
Right arrow Articles by Persing, D. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lodes, M. J.
Right arrow Articles by Persing, D. H.

 Previous Article  |  Next Article 

Infection and Immunity, May 2000, p. 2783-2790, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Serological Expression Cloning of Novel Immunoreactive Antigens of Babesia microti

Michael J. Lodes,1,* Raymond L. Houghton,1 Elizabeth S. Bruinsma,2 Raodoh Mohamath,1 Lisa D. Reynolds,1 Darin R. Benson,1 Peter J. Krause,3 Steven G. Reed,1,4 and David H. Persing2,dagger

Corixa Corporation1 and Infectious Disease Research Institute,4 Seattle, Washington; Department of Medicine and Pathology, Mayo Clinic, Rochester, Minnesota2; and University of Connecticut School of Medicine, Farmington, Connecticut3

Received 23 September 1999/Returned for modification 8 November 1999/Accepted 15 February 2000

Increased recognition of the prevalence of human babesiosis in the United States, together with rising concern about the potential for transmission of this infection by blood transfusion, has provided motivation to develop definitive serologic and molecular tests for the causative agent, Babesia microti. To develop more sensitive and specific assays for B. microti, we screened a genomic expression library with patient serum pools. This screening resulted in the identification of three classes of novel genes and an additional two novel, unrelated genes, which together encode a total of 17 unique B. microti antigens. The first class (BMN1-2 family) of genes encodes seven closely related antigens with a degenerate six-amino-acid repeat that shows limited homology to Plasmodium sp. merozoite and sporozoite surface antigens. A second class (BMN1-8 family) of genes encodes six related antigens, and the third class (BMN1-17 family) of genes encodes two related antigens. The two remaining genes code for novel and unrelated sequences. Among the three classes of antigens and remaining novel sequences, five were chosen to code for the most immunodominant antigens (BMN1-2, -9, -15, and -17 and MN-10). Western blot analysis with the resulting recombinant proteins indicated that these antigens were targets of humoral immune responses during B. microti infection in humans.

* Corresponding author. Mailing address: Corixa Corporation, 1124 Columbia St., Suite 200, Seattle, WA 98104. Phone: (206) 754-5797. Fax: (206) 754-5715. E-mail: lodes{at}corixa.com.

dagger Present address: Infectious Disease Research Institute and Corixa Corporation, Seattle, WA 98104.

Infection and Immunity, May 2000, p. 2783-2790, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Yu, W. L., Dan, H., Lin, M. (2007). Novel protein targets of the humoral immune response to Listeria monocytogenes infection in rabbits. J Med Microbiol 56: 888-895 [Abstract] [Full Text]  
  • Homer, M. J., Lodes, M. J., Reynolds, L. D., Zhang, Y., Douglass, J. F., McNeill, P. D., Houghton, R. L., Persing, D. H. (2003). Identification and Characterization of Putative Secreted Antigens from Babesia microti. J. Clin. Microbiol. 41: 723-729 [Abstract] [Full Text]  
  • Yokoyama, N., Bork, S., Nishisaka, M., Hirata, H., Matsuo, T., Inoue, N., Xuan, X., Suzuki, H., Sugimoto, C., Igarashi, I. (2003). Roles of the Maltese Cross Form in the Development of Parasitemia and Protection against Babesia microti Infection in Mice. Infect. Immun. 71: 411-417 [Abstract] [Full Text]  
  • Clawson, M. L., Paciorkowski, N., Rajan, T. V., La Vake, C., Pope, C., La Vake, M., Wikel, S. K., Krause, P. J., Radolf, J. D. (2002). Cellular Immunity, but Not Gamma Interferon, Is Essential for Resolution of Babesia microti Infection in BALB/c Mice. Infect. Immun. 70: 5304-5306 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»