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Infection and Immunity, May 2000, p. 2819-2826, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Role of Manganese-Containing Superoxide Dismutase in Oxidative Stress and Virulence of Streptococcus pneumoniae

Hasan Yesilkaya,1 Aras Kadioglu,1 Neill Gingles,1 Janet E. Alexander,1 Tim J. Mitchell,2 and Peter W. Andrew1,*

Department of Microbiology and Immunology, University of Leicester, Leicester LE1 9HN,1 and Division of Infection and Immunity, University of Glasgow, Glasgow G12 8QQ,2 United Kingdom

Received 12 April 1999/Returned for modification 22 June 1999/Accepted 27 December 1999

Streptococcus pneumoniae was shown to contain two types of superoxide dismutase, MnSOD and FeSOD. Levels of MnSOD increased during growth in an aerobic environment. The sodA gene, encoding MnSOD, of virulent S. pneumoniae type 2 strain D39 was inactivated to give mutant D39HY1. Aerobically, D39HY1 had a lower growth rate than the wild type and exhibited susceptibility to the redox-active compound paraquat, but anaerobic growth of D39HY1 was identical to that of the wild type. Virulence studies showed that the median survival time of mice infected intranasally with D39HY1 was significantly longer than that of mice infected with the wild-type pneumococcus. In contrast to the wild type, D39HY1 did not multiply in lungs during the first 24 h but thereafter grew at the same rate as the wild type. Appearance in the bloodstream was also delayed, but growth in the blood was unimpaired by the sodA mutation. The pattern of inflammation in lungs infected with D39HY1 differed from that in wild-type-infected mice. After infection with D39HY1, neutrophils were densely packed around bronchioles, in contrast to the wild-type infection, where neutrophils were more diffusely localized.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Medical Sciences Building, University of Leicester, University Rd., Leicester LE1 9HN, United Kingdom. Phone: (44-0116) 2525030. Fax: (44-116) 2522941. E-mail: pwa{at}leicester.ac.uk.

Infection and Immunity, May 2000, p. 2819-2826, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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