Pseudomonas aeruginosa Cystic Fibrosis Isolates Induce Rapid, Type III Secretion-Dependent, but ExoU-Independent, Oncosis of Macrophages and Polymorphonuclear Neutrophils

doi:10.1128/IAI.68.5.2916-2924.2000

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Infection and Immunity, May 2000, p. 2916-2924, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pseudomonas aeruginosa Cystic Fibrosis Isolates Induce Rapid, Type III Secretion-Dependent, but ExoU-Independent, Oncosis of Macrophages and Polymorphonuclear Neutrophils

Denis Dacheux,¹ Bertrand Toussaint,¹ Marceline Richard,² Guy Brochier,² Jacques Croize,³ and Ina Attree¹^,^*

Département de Biologie Moléculaire et Structurale, BBSI, UMR-314 CNRS, CEA-Grenoble,¹ and Service de Bactériologie et Virologie, CHU,³ Grenoble, and Service d'Imagerie et de Microscopie Electronique, CRSSA, La Tronche,² France

Received 18 November 1999/Returned for modification 27 December 1999/Accepted 25 January 2000

Pseudomonas aeruginosa, an opportunistic pathogen responsible most notably for severe infections in cystic fibrosis (CF) patients,utilizes the type III secretion system for eukaryotic cell intoxication.The CF clinical isolate CHA shows toxicity towards human polymorphonuclearneutrophils (PMNs) which is dependent on the type III secretionsystem but independent of the cytotoxin ExoU. In the present study,the cytotoxicity of this strain toward human and murine macrophageswas demonstrated. In low-multiplicity infections (multiplicityof infection, 10), approximately 40% of the cells die within 60min. Analysis of CHA-infected cells by transmission electron microscopy,DNA fragmentation assay, and Hoechst staining revealed the hallmarksof oncosis: cellular and nuclear swelling, disintegration of theplasma membrane, and absence of DNA fragmentation. A panel of29 P. aeruginosa CF isolates was screened for type III systemgenotype, protein secretion profile, and cytotoxicity toward PMNsand macrophages. This study showed that six CF isolates were ableto induce rapid ExoU-independent oncosis on phagocytecells.

^* Corresponding author. Mailing address: DBMS/BBSI, CEA-Grenoble, 17 rue des Martyrs, 38054 Grenoble cedex 09, France. Phone:33.4.76.88.34.83. Fax: 33.4.76.88.44.99. E-mail: iattreedelic{at}cea.fr.

Infection and Immunity, May 2000, p. 2916-2924, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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