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Infection and Immunity, May 2000, p. 2995-2997, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Biochemical and Genetic Evidence for Phospholipase C Activity in Mycobacterium ulcerans

Arley Gomez,1,2,* Armand Mve-Obiang,1,3 Bernard Vray,4 José Remacle,3 Karim Chemlal,1 Wayne M. Meyers,5 Françoise Portaels,1 and Pierre-Alain Fonteyne1

Mycobacteriology Unit, Institute of Tropical Medicine, B-2000 Antwerp,1 Laboratoire d'Immunologie Experimentale, Faculté de Médecine, Université Libre de Bruxelles, Brussels,4 and Laboratoire de Biochimie et Biologie Cellulaire, Facultés Universitaires Notre Dame de la Paix, B-5000 Namur,3 Belgium; Faculty of Medicine, Universidad del Quindio, Quindio, Colombia2; and Armed Forces Institute of Pathology, Washington, D.C. 20306-60005

Received 28 October 1999/Returned for modification 22 December 1999/Accepted 14 February 2000

This study reports the existence of phospholipase C and D enzymatic activities in Mycobacterium ulcerans cultures as determined by use of thin-layer chromatography to detect diglycerides in hydrolysates of radiolabeled phosphatidylcholine. M. ulcerans DNA sequences homologous to the genes encoding phospholipase C in Mycobacterium tuberculosis and Pseudomonas aeruginosa were identified by sequence analysis and DNA-DNA hybridization. Whether or not the phospholipase C and D enzymes of M. ulcerans plays a role in the pathogenesis of the disease needs further investigation.

* Corresponding author. Mailing address: Mycobacteriology Unit, Institute of Tropical Medicine. Nationalestraat 155, B-2000 Antwerp, Belgium. Phone: (323) 2476548. Fax: (323) 2476333. E-mail: arley{at}itg.be.

Infection and Immunity, May 2000, p. 2995-2997, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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