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Infection and Immunity, May 2000, p. 3015-3018, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Commercial Preparation of Catalase Inhibits Nitric Oxide Production by Activated Murine Macrophages: Role of Arginase

Y. Tian,1 Y. Xing,1 R. Magliozzo,2 K. Yu,1 B. R. Bloom,3 and J. Chan1,*

Departments of Medicine and Microbiology and Immunology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx,1 and Department of Chemistry, Brooklyn College, Brooklyn,2 New York, and The School of Public Health, Harvard Medical School, Boston, Massachusetts3

Received 8 September 1999/Returned for modification 21 October 1999/Accepted 14 February 2000

Catalase is widely used as a pharmacological probe to evaluate the role of hydrogen peroxide in antimicrobial activities of phagocytic cells. This report demonstrates that the ability of a commercial preparation of catalase to inhibit concomitantly macrophage antimycobacterial activity and production of reactive nitrogen intermediates can be attributed, at least in part, to the depletion of L-arginine by contaminating arginase. In experimental systems that employ pharmacological probes, the existence of nonspecific effects should be considered in data interpretation.

* Corresponding author. Mailing address: F406, Departments of Medicine and Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461. Phone: (718) 430-2678 or 920-7247. Fax: (718) 652-0536. E-mail: jchan{at}aecom.yu.edu.

Infection and Immunity, May 2000, p. 3015-3018, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Webb, J. L., Harvey, M. W., Holden, D. W., Evans, T. J. (2001). Macrophage Nitric Oxide Synthase Associates with Cortical Actin but Is Not Recruited to Phagosomes. Infect. Immun. 69: 6391-6400 [Abstract] [Full Text]  
  • Bekker, L.-G., Freeman, S., Murray, P. J., Ryffel, B., Kaplan, G. (2001). TNF-{{alpha}} Controls Intracellular Mycobacterial Growth by Both Inducible Nitric Oxide Synthase-Dependent and Inducible Nitric Oxide Synthase-Independent Pathways. J. Immunol. 166: 6728-6734 [Abstract] [Full Text]  


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