This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ogunniyi, A. D.
Right arrow Articles by Paton, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ogunniyi, A. D.
Right arrow Articles by Paton, J. C.

 Previous Article  |  Next Article 

Infection and Immunity, May 2000, p. 3028-3033, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Immunization of Mice with Combinations of Pneumococcal Virulence Proteins Elicits Enhanced Protection against Challenge with Streptococcus pneumoniae

A. David Ogunniyi,1 Rebekah L. Folland,1 David E. Briles,2 Susan K. Hollingshead,2 and James C. Paton1,*

Molecular Microbiology Unit, Women's and Children's Hospital, North Adelaide, SA 5006, Australia,1 and Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama2

Received 29 October 1999/Returned for modification 21 December 1999/Accepted 7 February 2000

The vaccine potential of a combination of three pneumococcal virulence proteins was evaluated in an active-immunization-intraperitoneal-challenge model in BALB/c mice, using very high challenge doses of Streptococcus pneumoniae. The proteins evaluated were a genetic toxoid derivative of pneumolysin (PdB), pneumococcal surface protein A (PspA), and a 37-kDa metal-binding lipoprotein referred to as PsaA. Mice immunized with individual proteins or combinations thereof were challenged with high doses of virulent type 2 or type 4 pneumococci. The median survival times for mice immunized with combinations of proteins, particularly PdB and PspA, were significantly longer than those for mice immunized with any of the antigens alone. A similar effect was seen in a passive protection model. Thus, combinations of pneumococcal proteins may provide the best non-serotype-dependent protection against S. pneumoniae.

* Corresponding author. Mailing address: Molecular Microbiology Unit, Women's and Children's Hospital, 72 King William Rd., North Adelaide, SA 5006, Australia. Phone: 61 8 8204 6302. Fax: 61 8 8204 6051. E-mail: patonj{at}wch.sa.gov.au.

Infection and Immunity, May 2000, p. 3028-3033, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lu, Y.-J., Forte, S., Thompson, C. M., Anderson, P. W., Malley, R. (2009). Protection against Pneumococcal Colonization and Fatal Pneumonia by a Trivalent Conjugate of a Fusion Protein with the Cell Wall Polysaccharide. Infect. Immun. 77: 2076-2083 [Abstract] [Full Text]  
  • Ogunniyi, A. D., Grabowicz, M., Mahdi, L. K., Cook, J., Gordon, D. L., Sadlon, T. A., Paton, J. C. (2009). Pneumococcal histidine triad proteins are regulated by the Zn2+-dependent repressor AdcR and inhibit complement deposition through the recruitment of complement factor H. FASEB J. 23: 731-738 [Abstract] [Full Text]  
  • Chiavolini, D., Pozzi, G., Ricci, S. (2008). Animal Models of Streptococcus pneumoniae Disease. Clin. Microbiol. Rev. 21: 666-685 [Abstract] [Full Text]  
  • Melin, M. M., Hollingshead, S. K., Briles, D. E., Lahdenkari, M. I., Kilpi, T. M., Kayhty, H. M. (2008). Development of Antibodies to PspA Families 1 and 2 in Children after Exposure to Streptococcus pneumoniae. CVI 15: 1529-1535 [Abstract] [Full Text]  
  • Melin, M. M., Hollingshead, S. K., Briles, D. E., Hanage, W. P., Lahdenkari, M., Kaijalainen, T., Kilpi, T. M., Kayhty, H. M. (2008). Distribution of Pneumococcal Surface Protein A Families 1 and 2 among Streptococcus pneumoniae Isolates from Children in Finland Who Had Acute Otitis Media or Were Nasopharyngeal Carriers. CVI 15: 1555-1563 [Abstract] [Full Text]  
  • Glover, D. T., Hollingshead, S. K., Briles, D. E. (2008). Streptococcus pneumoniae Surface Protein PcpA Elicits Protection against Lung Infection and Fatal Sepsis. Infect. Immun. 76: 2767-2776 [Abstract] [Full Text]  
  • Aranda, J., Garrido, M. E., Cortes, P., Llagostera, M., Barbe, J. (2008). Analysis of the Protective Capacity of Three Streptococcus suis Proteins Induced under Divalent-Cation-Limited Conditions. Infect. Immun. 76: 1590-1598 [Abstract] [Full Text]  
  • Mahdi, L. K., Ogunniyi, A. D., LeMessurier, K. S., Paton, J. C. (2008). Pneumococcal Virulence Gene Expression and Host Cytokine Profiles during Pathogenesis of Invasive Disease. Infect. Immun. 76: 646-657 [Abstract] [Full Text]  
  • Giefing, C., Meinke, A. L., Hanner, M., Henics, T., Minh, D. B., Gelbmann, D., Lundberg, U., Senn, B. M., Schunn, M., Habel, A., Henriques-Normark, B., Ortqvist, A., Kalin, M., von Gabain, A., Nagy, E. (2008). Discovery of a novel class of highly conserved vaccine antigens using genomic scale antigenic fingerprinting of pneumococcus with human antibodies. JEM 205: 117-131 [Abstract] [Full Text]  
  • Basset, A., Thompson, C. M., Hollingshead, S. K., Briles, D. E., Ades, E. W., Lipsitch, M., Malley, R. (2007). Antibody-Independent, CD4+ T-Cell-Dependent Protection against Pneumococcal Colonization Elicited by Intranasal Immunization with Purified Pneumococcal Proteins. Infect. Immun. 75: 5460-5464 [Abstract] [Full Text]  
  • Ogunniyi, A. D., LeMessurier, K. S., Graham, R. M. A., Watt, J. M., Briles, D. E., Stroeher, U. H., Paton, J. C. (2007). Contributions of Pneumolysin, Pneumococcal Surface Protein A (PspA), and PspC to Pathogenicity of Streptococcus pneumoniae D39 in a Mouse Model. Infect. Immun. 75: 1843-1851 [Abstract] [Full Text]  
  • Ogunniyi, A. D., Grabowicz, M., Briles, D. E., Cook, J., Paton, J. C. (2007). Development of a Vaccine against Invasive Pneumococcal Disease Based on Combinations of Virulence Proteins of Streptococcus pneumoniae. Infect. Immun. 75: 350-357 [Abstract] [Full Text]  
  • Zhang, Q., Bernatoniene, J., Bagrade, L., Paton, J. C., Mitchell, T. J., Hammerschmidt, S., Nunez, D. A., Finn, A. (2006). Regulation of production of mucosal antibody to pneumococcal protein antigens by T-cell-derived gamma interferon and interleukin-10 in children.. Infect. Immun. 74: 4735-4743 [Abstract] [Full Text]  
  • Morona, J. K., Morona, R., Paton, J. C. (2006). Attachment of capsular polysaccharide to the cell wall of Streptococcus pneumoniae type 2 is required for invasive disease. Proc. Natl. Acad. Sci. USA 103: 8505-8510 [Abstract] [Full Text]  
  • Hollingshead, S. K., Baril, L., Ferro, S., King, J., Coan, P., Briles, D. E., the Pneumococcal Proteins Epi Study Group, (2006). Pneumococcal surface protein A (PspA) family distribution among clinical isolates from adults over 50 years of age collected in seven countries. J Med Microbiol 55: 215-221 [Abstract] [Full Text]  
  • Jomaa, M., Yuste, J., Paton, J. C., Jones, C., Dougan, G., Brown, J. S. (2005). Antibodies to the Iron Uptake ABC Transporter Lipoproteins PiaA and PiuA Promote Opsonophagocytosis of Streptococcus pneumoniae. Infect. Immun. 73: 6852-6859 [Abstract] [Full Text]  
  • Trzcinski, K., Thompson, C., Malley, R., Lipsitch, M. (2005). Antibodies to Conserved Pneumococcal Antigens Correlate with, but Are Not Required for, Protection against Pneumococcal Colonization Induced by Prior Exposure in a Mouse Model. Infect. Immun. 73: 7043-7046 [Abstract] [Full Text]  
  • Yuste, J., Botto, M., Paton, J. C., Holden, D. W., Brown, J. S. (2005). Additive Inhibition of Complement Deposition by Pneumolysin and PspA Facilitates Streptococcus pneumoniae Septicemia. J. Immunol. 175: 1813-1819 [Abstract] [Full Text]  
  • Standish, A. J., Stroeher, U. H., Paton, J. C. (2005). The two-component signal transduction system RR06/HK06 regulates expression of cbpA in Streptococcus pneumoniae. Proc. Natl. Acad. Sci. USA 102: 7701-7706 [Abstract] [Full Text]  
  • Gor, D. O., Ding, X., Briles, D. E., Jacobs, M. R., Greenspan, N. S. (2005). Relationship between Surface Accessibility for PpmA, PsaA, and PspA and Antibody-Mediated Immunity to Systemic Infection by Streptococcus pneumoniae. Infect. Immun. 73: 1304-1312 [Abstract] [Full Text]  
  • Garmory, H. S., Titball, R. W. (2004). ATP-Binding Cassette Transporters Are Targets for the Development of Antibacterial Vaccines and Therapies. Infect. Immun. 72: 6757-6763 [Full Text]  
  • Kwon, H.-Y., Ogunniyi, A. D., Choi, M.-H., Pyo, S.-N., Rhee, D.-K., Paton, J. C. (2004). The ClpP Protease of Streptococcus pneumoniae Modulates Virulence Gene Expression and Protects against Fatal Pneumococcal Challenge. Infect. Immun. 72: 5646-5653 [Abstract] [Full Text]  
  • Johnston, J. W., Myers, L. E., Ochs, M. M., Benjamin, W. H. Jr., Briles, D. E., Hollingshead, S. K. (2004). Lipoprotein PsaA in Virulence of Streptococcus pneumoniae: Surface Accessibility and Role in Protection from Superoxide. Infect. Immun. 72: 5858-5867 [Abstract] [Full Text]  
  • Garcia-Suarez, M. d. M., Cima-Cabal, M. D., Florez, N., Garcia, P., Cernuda-Cernuda, R., Astudillo, A., Vazquez, F., De Los Toyos, J. R., Mendez, F. J. (2004). Protection against Pneumococcal Pneumonia in Mice by Monoclonal Antibodies to Pneumolysin. Infect. Immun. 72: 4534-4540 [Abstract] [Full Text]  
  • Preston, J.A., Beagley, K.W., Gibson, P.G., Hansbro, P.M. (2004). Genetic background affects susceptibility in nonfatal pneumococcal bronchopneumonia. Eur Respir J 23: 224-231 [Abstract] [Full Text]  
  • McCool, T. L., Cate, T. R., Tuomanen, E. I., Adrian, P., Mitchell, T. J., Weiser, J. N. (2003). Serum Immunoglobulin G Response to Candidate Vaccine Antigens during Experimental Human Pneumococcal Colonization. Infect. Immun. 71: 5724-5732 [Abstract] [Full Text]  
  • Kwon, H.-Y., Kim, S.-W., Choi, M.-H., Ogunniyi, A. D., Paton, J. C., Park, S.-H., Pyo, S.-N., Rhee, D.-K. (2003). Effect of Heat Shock and Mutations in ClpL and ClpP on Virulence Gene Expression in Streptococcus pneumoniae. Infect. Immun. 71: 3757-3765 [Abstract] [Full Text]  
  • Artz, A. S., Ershler, W. B., Longo, D. L. (2003). Pneumococcal Vaccination and Revaccination of Older Adults. Clin. Microbiol. Rev. 16: 308-318 [Abstract] [Full Text]  
  • Romero-Steiner, S., Pilishvili, T., Sampson, J. S., Johnson, S. E., Stinson, A., Carlone, G. M., Ades, E. W. (2003). Inhibition of Pneumococcal Adherence to Human Nasopharyngeal Epithelial Cells by Anti-PsaA Antibodies. CVI 10: 246-251 [Abstract] [Full Text]  
  • Roche, H., Hakansson, A., Hollingshead, S. K., Briles, D. E. (2003). Regions of PspA/EF3296 Best Able To Elicit Protection against Streptococcus pneumoniae in a Murine Infection Model. Infect. Immun. 71: 1033-1041 [Abstract] [Full Text]  
  • Zhang, Q., Choo, S., Finn, A. (2002). Immune Responses to Novel Pneumococcal Proteins Pneumolysin, PspA, PsaA, and CbpA in Adenoidal B Cells from Children. Infect. Immun. 70: 5363-5369 [Abstract] [Full Text]  
  • Gor, D. O., Ding, X., Li, Q., Schreiber, J. R., Dubinsky, M., Greenspan, N. S. (2002). Enhanced Immunogenicity of Pneumococcal Surface Adhesin A by Genetic Fusion to Cytokines and Evaluation of Protective Immunity in Mice. Infect. Immun. 70: 5589-5595 [Abstract] [Full Text]  
  • Ogunniyi, A. D., Giammarinaro, P., Paton, J. C. (2002). The genes encoding virulence-associated proteins and the capsule of Streptococcus pneumoniae are upregulated and differentially expressed in vivo. Microbiology 148: 2045-2053 [Abstract] [Full Text]  
  • Marra, A., Lawson, S., Asundi, J. S., Brigham, D., Hromockyj, A. E. (2002). In vivo characterization of the psa genes from Streptococcus pneumoniae in multiple models of infection. Microbiology 148: 1483-1491 [Abstract] [Full Text]  
  • Seo, J.-Y., Seong, S. Y., Ahn, B.-Y., Kwon, I. C., Chung, H., Jeong, S. Y. (2002). Cross-Protective Immunity of Mice Induced by Oral Immunization with Pneumococcal Surface Adhesin A Encapsulated in Microspheres. Infect. Immun. 70: 1143-1149 [Abstract] [Full Text]  
  • McCool, T. L., Cate, T. R., Moy, G., Weiser, J. N. (2002). The Immune Response to Pneumococcal Proteins during Experimental Human Carriage. JEM 195: 359-365 [Abstract] [Full Text]  
  • Khan, A. Q., Shen, Y., Wu, Z.-Q., Wynn, T. A., Snapper, C. M. (2002). Endogenous Pro- and Anti-Inflammatory Cytokines Differentially Regulate an In Vivo Humoral Response to Streptococcus pneumoniae. Infect. Immun. 70: 749-761 [Abstract] [Full Text]  
  • Brown, J. S., Ogunniyi, A. D., Woodrow, M. C., Holden, D. W., Paton, J. C. (2001). Immunization with Components of Two Iron Uptake ABC Transporters Protects Mice against Systemic Streptococcus pneumoniae Infection. Infect. Immun. 69: 6702-6706 [Abstract] [Full Text]  
  • Ogunniyi, A. D., Woodrow, M. C., Poolman, J. T., Paton, J. C. (2001). Protection against Streptococcus pneumoniae Elicited by Immunization with Pneumolysin and CbpA. Infect. Immun. 69: 5997-6003 [Abstract] [Full Text]  
  • Malley, R., Lipsitch, M., Stack, A., Saladino, R., Fleisher, G., Pelton, S., Thompson, C., Briles, D., Anderson, P. (2001). Intranasal Immunization with Killed Unencapsulated Whole Cells Prevents Colonization and Invasive Disease by Capsulated Pneumococci. Infect. Immun. 69: 4870-4873 [Abstract] [Full Text]  
  • Hollingshead, S. K., Becker, R., Briles, D. E. (2000). Diversity of PspA: Mosaic Genes and Evidence for Past Recombination in Streptococcus pneumoniae. Infect. Immun. 68: 5889-5900 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»