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Infection and Immunity, May 2000, p. 3053-3055, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evidence That Hyaluronidase Is Not Involved in Tissue Invasion of the Protozoan Parasite Entamoeba histolytica

Rosa Nickel,1 Robert Stern,2 and Matthias Leippe1,*

Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany,1 and Department of Pathology, School of Medicine, University of California, San Francisco, California 041432

Received 5 November 1999/Returned for modification 27 December 1999/Accepted 18 February 2000

As previous reports suggested that a hyaluronidase is involved in tissue invasion of Entamoeba histolytica, we searched for such an activity in trophozoite extracts. A hyaluronidase activity was not detectable in long-term cultures or in amoebae freshly passaged through a gerbil liver, as evidenced by four different techniques.

* Corresponding author. Mailing address: Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht St. 74, 20359 Hamburg, Germany. Phone: 49 40 42818 498. Fax: 49 40 42818 512. E-mail: leippe{at}bni.uni-hamburg.de.

Infection and Immunity, May 2000, p. 3053-3055, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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