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Infection and Immunity, June 2000, p. 3337-3343, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Immune Response to an 18-Kilodalton Outer Membrane Antigen Identifies Lipoprotein 20 as a Helicobacter pylori Vaccine Candidate

Jacqueline Keenan,1,* Jane Oliaro,2 Neil Domigan,3 Howard Potter,3 Geoff Aitken,4 Randall Allardyce,1 and Justin Roake1

Departments of Surgery,1 Pathology,3 and Medicine,4 Christchurch School of Medicine, Christchurch, and Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North,2 New Zealand

Received 13 December 1999/Returned for modification 18 January 2000/Accepted 1 March 2000

Experiments were performed using the standardized murine model of Helicobacter pylori infection to determine the immunogenicity of H. pylori outer membrane vesicles in immune protection. These vesicles, which are naturally shed from the surface of the bacterium, induce a protective response when administered intragastrically to mice in the presence of cholera holotoxin, despite the absence of the urease enzyme and associated Hsp54 chaperonin. Immunoblotting identified a specific serum immunoglobulin G (IgG) response to an 18-kDa outer membrane protein in a significant number of immunized animals. This commonly expressed, immunodominant protein was subsequently identified as lipoprotein 20 (Lpp20). Hybridoma backpacks secreting an IgG1 subclass monoclonal antibody to Lpp20 were generated in H. pylori-infected mice and were found to significantly reduce bacterial numbers, providing evidence that this surface-exposed antigen is a true vaccine candidate and not merely an antigenic marker for successful, protective immunization.

* Corresponding author. Mailing address: Department of Surgery, Christchurch School of Medicine, P.O. Box 4345, Christchurch, New Zealand. Phone: 64 3 3640 570. Fax: 64 3 3641 427. E-mail: jacqui.keenan{at}chmeds.ac.nz.

Infection and Immunity, June 2000, p. 3337-3343, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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