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Infection and Immunity, June 2000, p. 3431-3442, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Afa/Dr Diffusely Adhering Escherichia coli C1845 Infection Promotes Selective Injuries in the Junctional Domain of Polarized Human Intestinal Caco-2/TC7 Cells

Isabelle Peiffer,1 Anne-Béatrice Blanc-Potard,1 Marie-Françoise Bernet-Camard,1 Julie Guignot,1 Alain Barbat,2 and Alain L. Servin1,*

Unité 510, Institut National de la Santé et de la Recherche Médicale, Faculté de Pharmacie Paris XI, F-92296 Châtenay-Malabry,1 and Unité 504, Institut National de la Santé et de la Recherche Médicale, F-94807 Villejuif,2 France

Received 18 November 1999/Returned for modification 18 February 2000/Accepted 8 March 2000

The Afa/Dr diffusely adhering Escherichia coli (DAEC) C1845 strain harboring the F1845 fimbrial adhesin interacts with the brush border-associated CD55 molecule and promotes elongation of brush border microvilli resulting from rearrangement of the F-actin network. This phenomenon involves the activation of a cascade of signaling coupled to the glycosylphosphatidylinositol-anchored receptor of the F1845 adhesin. We provide evidence that infection of the polarized human intestinal cell line Caco-2/TC7 by strain C1845 is followed by an increase in the paracellular permeability for [3H]mannitol without a decrease of the transepithelial resistance of the monolayers. Alterations in the distribution of tight-junction (TJ)-associated occludin and ZO-1 protein are observed, whereas the distribution of the zonula adherens-associated E-cadherin is not affected. Using the recombinant E. coli strains HB101(pSSS1) and -(pSSS1C) expressing the F1845 fimbrial adhesin, we demonstrate that the adhesin-CD55 interaction is not sufficient for the induction of structural and functional TJ lesions. Moreover, using the actin filament-stabilizing agent Jasplakinolide, we demonstrate that the C1845-induced functional alterations in TJs are independent of the C1845-induced apical cytoskeleton rearrangements. The results indicated that pathogenic factor(s) other than F1845 adhesin may be operant in Afa/Dr DAEC C1845.

* Corresponding author. Mailing address: Unité 510 INSERM, Faculté de Pharmacie Paris XI, F-92296 Châtenay-Malabry, France. Phone and fax: 33.1.46.83.56.61. E-mail: alain.servin{at}cep.u-psud.fr.

Infection and Immunity, June 2000, p. 3431-3442, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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