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Infection and Immunity, June 2000, p. 3564-3568, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Recombinant Baculovirus-Expressed Plasmodium falciparum Receptor-Binding Domain of Erythrocyte Binding Protein EBA-175 Biologically Mimics Native Protein

Hong Liang, David L. Narum, Steven R. Fuhrmann, Tin Luu, and B. Kim Lee Sim*

EntreMed, Inc., Rockville, Maryland 20850

Received 31 January 2000/Accepted 16 March 2000

EBA-175 of Plasmodium falciparum is a merozoite ligand that binds its receptor glycophorin A on erythrocytes during invasion. The ligand-receptor interaction is dependent on sialic acids as well as the protein backbone of glycophorin A. Region II (RII) of EBA-175 has been defined as the receptor-binding domain. RII is divided into regions F1 and F2, which contain duplicated cysteine motifs. We expressed RII in a baculovirus and show that RII binds erythrocytes with a specificity identical to that of the native protein. We found that, consistent with the binding of erythrocytes to COS cells expressing F2, recombinant baculovirus-expressed F2 bound erythrocytes. About 20% of all baculovirus-expressed RII is N-glycosylated, unlike native P. falciparum proteins that remain essentially unglycosylated. However, glycosylation of recombinant RII did not affect its immunogenicity. Antibodies raised against both glycosylated and unglycosylated baculovirus-expressed RII recognized P. falciparum schizonts in immunofluorescence assays and also gave similar enzyme-linked immunosorbent assay titers. Furthermore, these antibodies have similar abilities to block native EBA-175 binding to erythrocytes. These results allow the development of RII as a vaccine candidate for preclinical assessment.

* Corresponding author. Mailing address: EntreMed, Inc., 9640 Medical Center Drive, Rockville, MD 20850. Phone: (301) 517-3307. Fax: (301) 294-1775. E-mail: kims{at}entremed.com.

Infection and Immunity, June 2000, p. 3564-3568, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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