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Infection and Immunity, June 2000, p. 3710-3715, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Interaction of Shigella flexneri IpaC with Model Membranes Correlates with Effects on Cultured Cells

Ngoc Tran,1 Alexa Barnoski Serfis,1 John C. Osiecki,2 Wendy L. Picking,2 Lisette Coye,3 Rebecca Davis,3 and William D. Picking2,*

Department of Chemistry1 and Department of Biology,3 Saint Louis University, St. Louis, Missouri, and Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas2

Received 20 December 1999/Returned for modification 4 February 2000/Accepted 3 March 2000

Invasion of enterocytes by Shigella flexneri requires the properly timed release of IpaB and IpaC at the host-pathogen interface; however, only IpaC has been found to possess quantifiable activities in vitro. We demonstrate here that when added to cultured cells, purified IpaC elicits cytoskeletal changes similar to those that occur during Shigella invasion. This IpaC effect may correlate with its ability to interact with model membranes at physiological pH and to promote entry by an ipaC mutant of S. flexneri.

* Corresponding author. Mailing address: Dept. of Molecular Biosciences, 8047 Haworth, University of Kansas, Lawrence, KS 66045. Phone: (785) 864-3299. Fax: (785) 864-5294. E-mail: picking{at}eagle.cc.ukans.edu.

Infection and Immunity, June 2000, p. 3710-3715, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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