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Infection and Immunity, June 2000, p. 3727-3730, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Stimulation of Bordetella pertussis Adenylate Cyclase Toxin Intoxication by Its Hemolysin Domain

Masaaki Iwaki,1,2,* Kazunari Kamachi,1 and Toshifumi Konda1

Department of Bacterial and Blood Products, National Institute of Infectious Diseases, Musashimurayama-shi, Tokyo 208-0011, Japan,1 and Unité de Biochimie des Régulations Cellulaires, Institut Pasteur, 75724 Paris Cedex 15, France2

Received 14 January 2000/Returned for modification 11 February 2000/Accepted 7 March 2000

The internalization of the N-terminal catalytic domain of Bordetella pertussis adenylate cyclase toxin (ACT) across the cytoplasmic membrane has been considered to occur independently from protein-protein interactions which can lead to oligomerization required for hemolytic activity by its C-terminal hemolysin domain. Here we report that when added in excess, this hemolysin domain stimulates the internalization, suggesting the involvement of protein-protein interactions in cell-invasive activity of ACT, as well as its hemolytic activity.

* Corresponding author. Mailing address: Department of Bacterial and Blood Products, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208-0011, Japan. Phone: 81-42-561-0771, ext. 363. Fax: 81-42-561-7173. E-mail: miwaki{at}nih.go.jp.

Infection and Immunity, June 2000, p. 3727-3730, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Vojtova-Vodolanova, J., Basler, M., Osicka, R., Knapp, O., Maier, E., Cerny, J., Benada, O., Benz, R., Sebo, P. (2009). Oligomerization is involved in pore formation by Bordetella adenylate cyclase toxin. FASEB J. 23: 2831-2843 [Abstract] [Full Text]  


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