Toll-Like Receptors Confer Responsiveness to Lipopolysaccharide from Porphyromonas gingivalis in Human Gingival Fibroblasts

doi:10.1128/IAI.68.6.3731-3735.2000

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Infection and Immunity, June 2000, p. 3731-3735, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Toll-Like Receptors Confer Responsiveness to Lipopolysaccharide from Porphyromonas gingivalis in Human Gingival Fibroblasts

Kouich Tabeta,¹ Kazuhisa Yamazaki,¹^,^* Sachiko Akashi,² Kensuke Miyake,² Hidefumi Kumada,³ Toshio Umemoto,³ and Hiromasa Yoshie¹

Department of Periodontology, Faculty of Dentistry, Niigata University, Niigata,¹ Department of Immunology, Saga Medical School, Saga,² and Department of Oral Microbiology, Kanagawa Dental College, Yokosuka,³ Japan

Received 24 September 1999/Returned for modification 1 December 1999/Accepted 29 February 2000

Gingival fibroblasts produce proinflammatory cytokines in response to lipopolysaccharide (LPS) from periodontopathic bacteria.Recently it has become evident that the human homologue of DrosophilaToll can transduce intracellular signaling by LPS stimulation.Toll-like receptors (TLRs) have been identified in myeloid cells;however, their role in nonmyeloid cells such as gingival fibroblastshas not been fully elucidated. Here, we report that human gingivalfibroblasts constitutively express TLR2 and TLR4 and that theirlevels of expression are increased by stimulation with LPS fromPorphyromonas gingivalis. Upregulated expression of interleukin-6gene and protein in fibroblasts stimulated with LPS is inhibitedby anti-TLR4 antibody. These findings suggest that TLRs may conferresponsiveness to LPS in gingivalfibroblasts.

^* Corresponding author. Mailing address: Department of Periodontology, Faculty of Dentistry, Niigata University, 5274, Gakkocho-Dori2-ban-cho, Niigata 951-8514, Japan. Phone: 81-25-227-2870. Fax:81-25-227-0808. E-mail: kaz{at}dent.niigata-u.ac.jp.

Infection and Immunity, June 2000, p. 3731-3735, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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