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Infection and Immunity, June 2000, p. 3731-3735, Vol. 68, No. 6
Department of Periodontology, Faculty of
Dentistry, Niigata University, Niigata,1
Department of Immunology, Saga Medical School,
Saga,2 and Department of Oral
Microbiology, Kanagawa Dental College,
Yokosuka,3 Japan
Received 24 September 1999/Returned for modification 1 December
1999/Accepted 29 February 2000
Gingival fibroblasts produce proinflammatory cytokines in response
to lipopolysaccharide (LPS) from periodontopathic bacteria. Recently it
has become evident that the human homologue of Drosophila Toll can transduce intracellular signaling by LPS stimulation. Toll-like receptors (TLRs) have been identified in myeloid cells; however, their role in nonmyeloid cells such as gingival fibroblasts has not been fully elucidated. Here, we report that human gingival fibroblasts constitutively express TLR2 and TLR4 and that their levels
of expression are increased by stimulation with LPS from Porphyromonas gingivalis. Upregulated expression of
interleukin-6 gene and protein in fibroblasts stimulated with LPS is
inhibited by anti-TLR4 antibody. These findings suggest that TLRs may
confer responsiveness to LPS in gingival fibroblasts.
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Toll-Like Receptors Confer Responsiveness to Lipopolysaccharide
from Porphyromonas gingivalis in Human Gingival
Fibroblasts
*
Corresponding author. Mailing address: Department of
Periodontology, Faculty of Dentistry, Niigata University, 5274, Gakkocho-Dori 2-ban-cho, Niigata 951-8514, Japan. Phone:
81-25-227-2870. Fax: 81-25-227-0808. E-mail:
kaz{at}dent.niigata-u.ac.jp.
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