This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rhem, M. N.
Right arrow Articles by Wilhelmus, K. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rhem, M. N.
Right arrow Articles by Wilhelmus, K. R.

 Previous Article  |  Next Article 

Infection and Immunity, June 2000, p. 3776-3779, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Collagen-Binding Adhesin Is a Virulence Factor in Staphylococcus aureus Keratitis

Marcus N. Rhem,1 Elizabeth M. Lech,1 Joseph M. Patti,2,dagger Damien McDevitt,2,Dagger Magnus Höök,2 Dan B. Jones,1 and Kirk R. Wilhelmus1,*

Sid W. Richardson Ocular Microbiology Laboratory, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine,1 and Center for Extracellular Matrix Biology, Albert B. Alkek Institute of Biosciences and Technology, Texas A&M University,2 Houston, Texas

Received 24 January 2000/Returned for modification 1 March 2000/Accepted 23 March 2000

A collagen-binding strain of Staphylococcus aureus produced suppurative inflammation in a rabbit model of soft contact lens-associated bacterial keratitis more often than its collagen-binding-negative isogenic mutant. Reintroduction of the cna gene on a multicopy plasmid into the mutant helped it regain its corneal adherence and infectivity. The topical application of a collagen-binding peptide before bacterial challenge decreased S. aureus adherence to deepithelialized corneas. These data suggest that the collagen-binding adhesin is involved in the pathogenesis of S. aureus infection of the cornea.

* Corresponding author. Mailing address: 6565 Fannin St., Suite NC205, Houston, TX 77030. Phone: (713) 798-5952. Fax: (713) 798-4142. E-mail: kirkw{at}bcm.tmc.edu.

dagger Present address: Inhibitex, Inc., Alpharetta, GA 30004.

Dagger Present address: Microbiology Department, SmithKline Beecham, Collegeville, PA 19426.

Infection and Immunity, June 2000, p. 3776-3779, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nallapareddy, S. R., Singh, K. V., Murray, B. E. (2008). Contribution of the Collagen Adhesin Acm to Pathogenesis of Enterococcus faecium in Experimental Endocarditis. Infect. Immun. 76: 4120-4128 [Abstract] [Full Text]  
  • Itoi, Y., Horinaka, M., Tsujimoto, Y., Matsui, H., Watanabe, K. (2006). Characteristic Features in the Structure and Collagen-Binding Ability of a Thermophilic Collagenolytic Protease from the Thermophile Geobacillus collagenovorans MO-1.. J. Bacteriol. 188: 6572-6579 [Abstract] [Full Text]  
  • Bannam, T. L., Teng, W. L., Bulach, D., Lyras, D., Rood, J. I. (2006). Functional Identification of Conjugation and Replication Regions of the Tetracycline Resistance Plasmid pCW3 from Clostridium perfringens. J. Bacteriol. 188: 4942-4951 [Abstract] [Full Text]  
  • Fulcher, R. A., Cole, L. E., Janowicz, D. M., Toffer, K. L., Fortney, K. R., Katz, B. P., Orndorff, P. E., Spinola, S. M., Kawula, T. H. (2006). Expression of Haemophilus ducreyi Collagen Binding Outer Membrane Protein NcaA Is Required for Virulence in Swine and Human Challenge Models of Chancroid.. Infect. Immun. 74: 2651-2658 [Abstract] [Full Text]  
  • Kreikemeyer, B., Nakata, M., Oehmcke, S., Gschwendtner, C., Normann, J., Podbielski, A. (2005). Streptococcus pyogenes Collagen Type I-binding Cpa Surface Protein: EXPRESSION PROFILE, BINDING CHARACTERISTICS, BIOLOGICAL FUNCTIONS, AND POTENTIAL CLINICAL IMPACT. J. Biol. Chem. 280: 33228-33239 [Abstract] [Full Text]  
  • Xu, Y., Liang, X., Chen, Y., Koehler, T. M., Hook, M. (2004). Identification and Biochemical Characterization of Two Novel Collagen Binding MSCRAMMs of Bacillus anthracis. J. Biol. Chem. 279: 51760-51768 [Abstract] [Full Text]  
  • Esmay, P. A., Billington, S. J., Link, M. A., Songer, J. G., Jost, B. H. (2003). The Arcanobacterium pyogenes Collagen-Binding Protein, CbpA, Promotes Adhesion to Host Cells. Infect. Immun. 71: 4368-4374 [Abstract] [Full Text]  
  • Calabi, E., Calabi, F., Phillips, A. D., Fairweather, N. F. (2002). Binding of Clostridium difficile Surface Layer Proteins to Gastrointestinal Tissues. Infect. Immun. 70: 5770-5778 [Abstract] [Full Text]  
  • Jett, B. D., Gilmore, M. S. (2002). Internalization of Staphylococcus aureus by Human Corneal Epithelial Cells: Role of Bacterial Fibronectin-Binding Protein and Host Cell Factors. Infect. Immun. 70: 4697-4700 [Abstract] [Full Text]  
  • Bjerketorp, J., Nilsson, M., Ljungh, A., Flock, J.-I., Jacobsson, K., Frykberg, L. (2002). A novel von Willebrand factor binding protein expressed by Staphylococcus aureus. Microbiology 148: 2037-2044 [Abstract] [Full Text]  
  • Moreau, J. M., Girgis, D. O., Hume, E. B. H., Dajcs, J. J., Austin, M. S., O'Callaghan, R. J. (2001). Phospholipase A2 in Rabbit Tears: A Host Defense against Staphylococcus aureus. IOVS 42: 2347-2354 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»