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Infection and Immunity, July 2000, p. 3894-3899, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Lipopolysaccharide Structures of Salmonella enterica Serovar Typhimurium and Neisseria gonorrhoeae Determine the Attachment of Human Mannose-Binding Lectin to Intact Organisms

Marina Devyatyarova-Johnson,1 Ian H. Rees,2 Brian D. Robertson,2 Malcolm W. Turner,1 Nigel J. Klein,1 and Dominic L. Jack1,*

Immunobiology Unit, Institute of Child Health, London WC1N 1EH,1 and Department of Infectious Diseases and Microbiology, Imperial College School of Medicine, St. Mary's Campus, London W2 1PG,2 United Kingdom

Received 27 January 2000/Returned for modification 13 March 2000/Accepted 6 April 2000

Mannose-binding lectin (MBL) is an important component of the innate immune system. It binds to the arrays of sugars commonly presented by microorganisms and activates the complement system independently of antibody. Despite detailed knowledge of the stereochemical basis of MBL binding, relatively little is known about how bacterial surface structures influence binding of the lectin. Using flow cytometry, we have measured the binding of MBL to a range of mutants of Salmonella enterica serovar Typhimurium and Neisseria gonorrhoeae which differ in the structure of expressed lipopolysaccharide (LPS). For both organisms, the possession of core LPS structures led to avid binding of MBL, which was abrogated by the addition of O antigen (Salmonella serovar Typhimurium) or sialic acid (N. gonorrhoeae). Truncation of the LPS within the core led to lower levels of MBL binding. It was not possible to predict the magnitude of MBL binding from the identity of the LPS terminal sugar alone, indicating that the three-dimensional disposition of LPS molecules is probably also of importance in determining MBL attachment. These results further support the hypothesis that LPS structure is a major determinant of MBL binding.

* Corresponding author. Present address: Division of Molecular and Genetic Medicine, F Floor, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX, United Kingdom. Phone: 44 (0114) 271 2968. Fax: 44 (0114) 273 9926. E-mail: D.L.Jack{at}sheffield.ac.uk.

Infection and Immunity, July 2000, p. 3894-3899, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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