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Infection and Immunity, July 2000, p. 4312-4318, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Antibodies against Ribosomal Phosphoprotein P0 of Plasmodium falciparum Protect Mice against Challenge with Plasmodium yoelii

Sanchita Chatterjee,1 Subhash Singh,1 Rashmi Sohoni,1 Nevil J. Singh,1,dagger Akhil Vaidya,2 Carole Long,2,Dagger and Shobhona Sharma1,*

Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400 005, India,1 and Department of Microbiology and Immunology, MCP-Hahnemann University, Philadelphia, Pennsylvania2

Received 8 February 2000/Returned for modification 16 March 2000/Accepted 23 April 2000

Antibodies against the Plasmodium falciparum P0 ribosomal phosphoprotein (PfP0) have been detected exclusively but extensively in malaria-immune persons. Polyclonal rabbit and mice sera were raised against two recombinant polypeptides of P. falciparum P0 protein, PfP0N and PfP0C, covering amino acids 17 to 61 and the remaining amino acids 61 to 316, respectively. Sera against both these domains detected a 35-kDa protein from Plasmodium yoelii subsp. yoelii, a rodent malarial parasite, and stained the surface of merozoites in immunofluorescence assays. Total immunoglobulin G (IgG) purified from rabbit and mouse anti-PfP0 sera by ammonium sulfate and DEAE-cellulose chromatography was used for passive transfer experiments in mice. Mice passively immunized with both anti-PfP0N and anti-PfP0C showed distinctly lower levels of parasitemia than control mice. With immunizations on days -1, 0, 1, 3, and 5, about 50% of both sets of mice receiving anti-PfP0N and anti-PfP0C cleared the lethal 17XL strain of P. yoelii and revived by day 25. All the control mice died by day 10. By extending the immunization schedule, the survival period of the mice could be extended for every mouse that received anti-PfP0 IgG. These data demonstrate the cross-protection of the anti-PfP0 IgG and establish parasite P0 protein as a target for invasion-blocking antibodies.

* Corresponding author. Mailing address: Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India. Phone: 091-22-215-2971/2979, extn. 2570. Fax: 091-22-215-2110/2181. E-mail: sharma{at}tifr.res.in.

dagger Present address: LCMI-NIAID, National Institutes of Health, Bethesda, MD 20892.

Dagger Present address: Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892.

Infection and Immunity, July 2000, p. 4312-4318, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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