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Infection and Immunity, July 2000, p. 4319-4322, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
The Entamoeba histolytica Mitochondrion-Derived
Organelle (Crypton) Contains Double-Stranded DNA and Appears To Be
Bound by a Double Membrane
Sudip
Ghosh,1
Jessica
Field,1
Rick
Rogers,2
Mark
Hickman,3 and
John
Samuelson1,*
Department of Immunology and Infectious
Diseases,1 BioMedical Imaging
Institute,2 and Department of Cancer
Cell Biology,3 Harvard School of Public Health,
Boston, Massachusetts 02115
Received 2 December 1999/Returned for modification 24 January
2000/Accepted 19 March 2000
Amebae have an Hsp60-associated, mitochondrion-derived organelle
(crypton). In this study, the crypton was stained with multiple DNA-binding fluorochromes and a monoclonal anti-double-stranded DNA
antibody. Transmission microscopy of partially purified cryptons revealed organelles bound by a double membrane.
*
Corresponding author. Mailing address: Department of
Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Ave., Boston, MA 02115. Phone: (617) 432-4670. Fax:
(617) 738-4914. E-mail: jsamuels{at}hsph.harvard.edu.
Infection and Immunity, July 2000, p. 4319-4322, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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