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Infection and Immunity, July 2000, p. 4319-4322, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Entamoeba histolytica Mitochondrion-Derived Organelle (Crypton) Contains Double-Stranded DNA and Appears To Be Bound by a Double Membrane

Sudip Ghosh,1 Jessica Field,1 Rick Rogers,2 Mark Hickman,3 and John Samuelson1,*

Department of Immunology and Infectious Diseases,1 BioMedical Imaging Institute,2 and Department of Cancer Cell Biology,3 Harvard School of Public Health, Boston, Massachusetts 02115

Received 2 December 1999/Returned for modification 24 January 2000/Accepted 19 March 2000

Amebae have an Hsp60-associated, mitochondrion-derived organelle (crypton). In this study, the crypton was stained with multiple DNA-binding fluorochromes and a monoclonal anti-double-stranded DNA antibody. Transmission microscopy of partially purified cryptons revealed organelles bound by a double membrane.


* Corresponding author. Mailing address: Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Ave., Boston, MA 02115. Phone: (617) 432-4670. Fax: (617) 738-4914. E-mail: jsamuels{at}hsph.harvard.edu.


Infection and Immunity, July 2000, p. 4319-4322, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



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