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Infection and Immunity, July 2000, p. 4349-4353, Vol. 68, No. 7
Laboratório de Fisiologia Celular, Instituto de
Biofisica Carlos Chagas Filho, CCS, Universidade Federal do Rio de
Janeiro, 21941-590 Rio de Janeiro,1
Departamento de Biofisica e Biometria, Instituto de Biologia,
Universidade do Estado do Rio de Janeiro, 20551-030 Rio de
Janeiro,2 and Departamento de
Genética, Instituto de Biologia, CCS, Universidade Federal do
Rio de Janeiro, 21944-970 Rio de Janeiro,3 Brazil,
and Department of Microbiology and Immunology, Tulane
University Medical Center, School of Medicine, New Orleans,
Louisiana 701124
Received 28 December 1999/Returned for modification 10 February
2000/Accepted 4 April 2000
Systemic and mucosal antibody responses against both the major
subunit of colonization factor antigen I (CFA/I) of enterotoxigenic Escherichia coli (ETEC) and the somatic lipopolysaccharide
expressed by recombinant bivalent Salmonella vaccine
strains were significantly enhanced by coadministration of a detoxified
derivative with preserved adjuvant effects of the ETEC heat-labile
toxin, LT(R192G). The results further support the adjuvant
effects of LT(R192G) and represent a simple alternative to
improve responses against passenger antigens expressed by orally
delivered Salmonella vaccine strains.
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Adjuvant Activity of a Nontoxic Mutant of Escherichia
coli Heat-Labile Enterotoxin on Systemic and Mucosal Immune
Responses Elicited against a Heterologous Antigen Carried by a Live
Salmonella enterica Serovar Typhimurium Vaccine
Strain
*
Corresponding author. Present address: Departamento de
Microbiologia, Universidade de São Paulo, Institute of Biomedical Sciences-ICB II, Av. Prof. Lineu Prestes, 1374-1st Fl. São
Paulo, 05508-900, Brazil. Phone: 5521-560-8093. Fax: 5521-280-8193. E-mail: lcsf{at}biof.ufrj.br.
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