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Infection and Immunity, July 2000, p. 4363-4367, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cytocidal and Apoptotic Effects of the ClyA Protein from Escherichia coli on Primary and Cultured Monocytes and Macrophages

Xin-He Lai,1,2,dagger Ignacio Arencibia,3 Anders Johansson,4 Sun Nyunt Wai,1,5 Jan Oscarsson,1 Sotos Kalfas,4 Karl-Gösta Sundqvist,3 Yoshimitsu Mizunoe,5 Anders Sjöstedt,2 and Bernt Eric Uhlin1,*

Departments of Microbiology,1 Clinical Bacteriology,2 Clinical Immunology,3 and Oral Biology,4 Umeå University, S-901 87 Umeå, Sweden, and Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan5

Received 4 November 1999/Returned for modification 3 February 2000/Accepted 1 April 2000

Cytolysin A (ClyA) is a newly discovered cytolytic protein of Escherichia coli K-12 that mediates a hemolytic phenotype. We show here that highly purified ClyA and ClyA-expressing E. coli were cytotoxic and apoptogenic to fresh as well as cultured human and murine monocytes/macrophages.

* Corresponding author. Mailing address: Department of Microbiology, Umeå University, S-901 87 Umeå, Sweden. Phone: 46 90 785 6731. Fax: 46 90 772630. E-mail: bernt.eric.uhlin{at}micro.umu.se.

dagger Permanent address: Priority Laboratory of Molecular Medical Bacteriology of Ministry of Public Health, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing, China.

Infection and Immunity, July 2000, p. 4363-4367, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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