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Infection and Immunity, August 2000, p. 4391-4398, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

beta -1,2-Linked Oligomannosides from Candida albicans Bind to a 32-Kilodalton Macrophage Membrane Protein Homologous to the Mammalian Lectin Galectin-3

Chantal Fradin, Daniel Poulain, and Thierry Jouault*

Laboratoire de Mycologie Fondamentale et Appliquée, INSERM E9915, Faculté de Médecine, Pôle Recherche, 59037 Lille Cedex, France

Received 31 January 2000/Returned for modification 10 March 2000/Accepted 26 April 2000

beta -1,2-linked oligomannoside residues are present, associated with mannan and a glycolipid, the phospholipomannan, at the Candida albicans cell wall surface. beta -1,2-linked oligomannoside residues act as adhesins for macrophages and stimulate these cells to undergo cytokine production. To characterize the macrophage receptor involved in the recognition of C. albicans beta -1,2-oligomannoside we used the J774 mouse cell line, which is devoid of the receptor specific for alpha -linked mannose residues. A series of experiments based on affinity binding on either C. albicans yeast cells or beta -1,2-oligomannoside-conjugated bovine serum albumin (BSA) and subsequent disclosure with biotinylated conjugated BSA repeatedly led to the detection of a 32-kDa macrophage protein. An antiserum specific for this 32-kDa protein inhibited C. albicans binding to macrophages and was used to immunoprecipitate the molecule. Two high-pressure liquid chromatography-purified peptides from the 32-kDa tryptic digest showed complete homology to galectin-3 (previously designated Mac-2 antigen), an endogenous lectin with pleiotropic functions which is expressed in a wide variety of cell types with which C. albicans interacts as a saprophyte or a parasite.

* Corresponding author. Mailing address: Laboratoire de Mycologie Fondamentale et Appliquée, INSERM E9915, Faculté de Médecine, Pôle Recherche, Place Verdun, 59037 Lille Cedex, France. Phone: 33 3 20 47 26 29. Fax: 33 3 20 47 26 25. E-mail: tjou{at}worldnet.fr.

Infection and Immunity, August 2000, p. 4391-4398, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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