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Infection and Immunity, August 2000, p. 4477-4484, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Depressed Interleukin-12 (IL-12), but not IL-18, Production in Response to a 30- or 32-Kilodalton Mycobacterial Antigen in Patients with Active Pulmonary Tuberculosis

Chang-Hwa Song,1 Hwa-Jung Kim,1 Jeong-Kyu Park,1 Jae-Hyun Lim,1 Un-Ok Kim,1 Jun-Sang Kim,2 Tae-Hyun Paik,3 Kyung-Jin Kim,4 Ji-Won Suhr,5 and Eun-Kyeong Jo1,*

Department of Microbiology1 and Department of Therpeutic Radiology,2 College of Medicine, Chungnam National University, Taejon 301-131, Department of Microbiology,3 and Department of Obstetrics and Gynecology,4 College of Medicine, Konyang University, Nonsan, Chungnam 320-711, and Department of Internal Medicine, Catholic University, Taejon 301-723,5 Korea

Received 3 December 1999/Returned for modification 18 February 2000/Accepted 5 May 2000

The secreted 30-kDa antigen (Ag) of Mycobacterium tuberculosis directly stimulates Th1-type protective cytokine responses in healthy tuberculin reactors but not in patients with active tuberculosis (TB). To examine the cytokine profiles attributable to Th1 suppression associated with active TB, interleukin-12 (IL-12), IL-18, and IL-10 production in response to a 30- or 32-kDa Ag in 16 patients with active pulmonary TB and 24 healthy controls was investigated by enzyme-linked immunosorbent assay. In TB patients, production of IL-12 p40, as well as gamma interferon (IFN-gamma ), by 30- or 32-kDa Ag-stimulated peripheral blood mononuclear cells (PBMC) was significantly decreased compared with that in healthy tuberculin reactors. There were no significant differences in IL-18 production between patients and controls early during stimulation (16 h). However, PBMC from patients showed significantly enhanced IL-18 proteins after 96 h of stimulation. Similarly, higher IL-10 production was observed in the TB patients than in healthy tuberculin reactors. After 2 months of anti-TB therapy, the mean IFN-gamma and IL-12 p40 production and the mean blastogenic responses were significantly increased in PBMC in the 10 TB patients who were followed up. Our findings provide evidence that depressed IL-12 in response to the 30- or 32-kDa Ag is involved in the immunopathogenesis of human active pulmonary TB.

* Corresponding author. Mailing address: Department of Microbiology, School of Medicine, Chungnam National University, 6 Munhwa-dong, Chung-ku, Taejon 301-131, Korea. Phone: 82-42-580-8243. Fax: 82-42-585-3686. E-mail: hayoungj{at}hanbat.chungnam.ac.kr.

Infection and Immunity, August 2000, p. 4477-4484, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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