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Infection and Immunity, August 2000, p. 4585-4592, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Role of Pulmonary Alveolar Macrophages in Defense of the Lung against Pseudomonas aeruginosa

Dorothy O. Y. Cheung,1 Keith Halsey,2 and David P. Speert1,2,*

Department of Pathology and Laboratory Medicine1 and Division of Infectious and Immunological Diseases, Department of Pediatrics,2 University of British Columbia, Vancouver, British Columbia, Canada

Received 1 March 2000/Returned for modification 28 March 2000/Accepted 17 May 2000

Alveolar macrophages (AM) provide one of the first lines of defense against microbial invasion in the lower airways. The role of AM in the clearance of Pseudomonas aeruginosa in mice after intrapulmonary challenge was evaluated. AM were depleted by intranasal administration of liposome-encapsulated dichloromethylene diphosphonate. At 24 h following the instillation of liposomes, a sublethal dose of P. aeruginosa was inoculated intranasally. Spleen, liver, and lung tissue was then evaluated for viable bacteria and for histopathology. AM depletion of 78 to 88% did not affect the survival rate of infected mice or clearance of P. aeruginosa from the spleen, liver, or lung, compared to the control group, but the mice's susceptibility to Klebsiella pneumoniae was greatly enhanced. The recruitment of neutrophils to the lung was also not affected. Freshly explanted AM were not competent to phagocytose unopsonized P. aeruginosa but were able to phagocytose zymosan particles. Further studies were conducted to assess the in situ phagocytic activities of AM. Three hours after the intranasal instillation of P. aeruginosa or other particles, bronchoalveolar lavage was performed. AM phagocytosis of zymosan particles and latex beads exceeded that of P. aeruginosa. Neutrophils were recruited to the lung in response to a high-dose bacterial challenge. These results suggest that AM do not play an important role in defense of the lung against P. aeruginosa.

* Corresponding author. Mailing address: Room 377, Research Centre, 950 West 28th Ave., Vancouver, BC, V5Z 4H4, Canada. Phone: (604) 875-2438. Fax: (604) 875-2226. E-mail: speert{at}interchange.ubc.ca.

Infection and Immunity, August 2000, p. 4585-4592, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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