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Infection and Immunity, August 2000, p. 4673-4680, Vol. 68, No. 8
Department of Biological Sciences, University
of Wollongong, New South Wales, Australia,1 and
Department of Microbial Pathogenicity and Vaccine Research,
Division of Microbiology, GBF-National Research Centre for
Biotechnology, Braunschweig, Germany2
Received 8 November 1999/Returned for modification 3 January
2000/Accepted 5 May 2000
The phosphoglucomutase (PGM)-encoding gene of Bordetella
bronchiseptica is required for lipopolysaccharide (LPS)
biosynthesis. An insertion mutant of the wild-type B. bronchiseptica strain BB7865 which disrupted LPS biosynthesis was
created and characterized (BB7865pgm). Genetic analysis of
the mutated gene showed it shares high identity with PGM genes of
various bacterial species and forms part of an operon which also
encompasses the gene encoding phosphoglucose isomerase. Functional
assays for PGM revealed that enzyme activity is expressed in both
bvg-positive and bvg-negative strains of
B. bronchiseptica and is substantially reduced in
BB7865pgm. Complementation of the mutated PGM gene with
that from BB7865 restored the wild-type condition for all
phenotypes tested. The ability of the mutant BB7865pgm to
survive within J774.A1 cells was significantly reduced at 2 h
(40% reduction) and 24 h (56% reduction) postinfection.
BB7865pgm was also significantly attenuated in its ability
to survive in vivo following intranasal infection of mice, being
effectively cleared from the lungs within 4 days, whereas the wild-type
strain persisted at least 35 days. The activities of superoxide
dismutase, urease, and acid phosphatase were unaffected in the
PGM-deficient strain. In contrast, the inability to produce wild-type
LPS resulted in a reduced bacterial resistance to oxidative stress and
a higher susceptibility to the antimicrobial peptide cecropin P.
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Role of Phosphoglucomutase of Bordetella
bronchiseptica in Lipopolysaccharide Biosynthesis and
Virulence
*
Corresponding author. Mailing address: Department of
Biological Sciences, University of Wollongong, New South Wales,
Australia 2522. Phone: 0061-242213439. Fax: 0061-242214135. E-mail:
mwalker{at}uow.edu.au.
Present address: Endocrine Unit, Department of Medicine, John
Hunter Hospital, Newcastle, New South Wales, Australia.
Infection and Immunity, August 2000, p. 4673-4680, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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