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Infection and Immunity, August 2000, p. 4759-4764, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Decorin-Binding Protein A (DbpA) of Borrelia burgdorferi Is Not Protective When Immunized Mice Are Challenged via Tick Infestation and Correlates with the Lack of DbpA Expression by B. burgdorferi in Ticks

Kayla E. Hagman,1 Xiaofeng Yang,1 Stephen K. Wikel,2 George B. Schoeler,2 Melissa J. Caimano,3 Justin D. Radolf,3,4 and Michael V. Norgard1,*

Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 752351; Oklahoma State University, Stillwater, Oklahoma 740782; and Center for Microbial Pathogenesis3 and Department of Medicine,4 University of Connecticut Health Center, Farmington, Connecticut 06030

Received 23 March 2000/Returned for modification 2 May 2000/Accepted 13 May 2000

Previous studies showed that decorin-binding protein A (DbpA) of Borrelia burgdorferi was a protective immunogen in the murine model of Lyme borreliosis when mice were challenged (needle inoculated) intradermally with in vitro-cultivated spirochetes. In the present study, DbpA-immunized C3H/HeJ mice were not protected from infection when infested with Ixodes scapularis nymphs harboring virulent B. burgdorferi 297. This lack of protection correlated with the failure to detect DbpA on B. burgdorferi in ticks, suggesting that DbpA is not available as a target for bactericidal antibodies in serum when B. burgdorferi-infected ticks take their blood meal from an immunized host. The failure of DbpA immunization to protect tick-challenged mice contradicts the results of earlier needle inoculation vaccination experiments and suggests that DbpA may not be suitable as a Lyme disease vaccine.

* Corresponding author. Mailing address: Department of Microbiology, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, TX 75235-9048. Phone: (214) 648-5900. Fax: (214) 648-5905. E-mail: norgard{at}utsw.swmed.edu.

Infection and Immunity, August 2000, p. 4759-4764, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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