Infection and Immunity, August 2000, p. 4773-4777, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Department of Biochemistry1 and Oral Health Science Center,2 Tokyo Dental College, Mihama-ku, Chiba City 261-8502, and Department of Oral Biochemistry, School of Dentistry, Tohoku University, Aoba-ku, Sendai City 980-8575,3 Japan
Received 10 January 2000/Returned for modification 6 March 2000/Accepted 26 April 2000
The act gene was identified and an act mutant as well as the pfl mutant was constructed in Streptococcus mutans. Pyruvate formate-lyase (PFL) activity was regenerated with the mixture of the respective cell extracts from these mutants by complementary reconstitution of the in vitro reactivating system. The S. mutans act gene encoded the sole enzyme able to activate the PFL protein in this organism.
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