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Infection and Immunity, August 2000, p. 4773-4777, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of the Streptococcus mutans Pyruvate Formate-Lyase (PFL)-Activating Enzyme Gene by Complementary Reconstitution of the In Vitro PFL-Reactivating System

Yasuhito Yamamoto,1,* Yutaka Sato,1,2 Shoko Takahashi-Abbe,3 Nobuhiro Takahashi,3 and Harutoshi Kizaki1,2

Department of Biochemistry1 and Oral Health Science Center,2 Tokyo Dental College, Mihama-ku, Chiba City 261-8502, and Department of Oral Biochemistry, School of Dentistry, Tohoku University, Aoba-ku, Sendai City 980-8575,3 Japan

Received 10 January 2000/Returned for modification 6 March 2000/Accepted 26 April 2000

The act gene was identified and an act mutant as well as the pfl mutant was constructed in Streptococcus mutans. Pyruvate formate-lyase (PFL) activity was regenerated with the mixture of the respective cell extracts from these mutants by complementary reconstitution of the in vitro reactivating system. The S. mutans act gene encoded the sole enzyme able to activate the PFL protein in this organism.

* Corresponding author. Mailing address: Department of Biochemistry, Tokyo Dental College, Masago 1-2-2, Mihama-ku, Chiba City 261-8502, Japan. Phone: 81-43-270-3750. Fax: 81-43-270-3752. E-mail: yayamamo{at}tdc.ac.jp.

Infection and Immunity, August 2000, p. 4773-4777, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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