This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wilson, R. L.
Right arrow Articles by Jones, B. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wilson, R. L.
Right arrow Articles by Jones, B. D.

 Previous Article  |  Next Article 

Infection and Immunity, August 2000, p. 4782-4785, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Salmonella enterica Serovars Gallinarum and Pullorum Expressing Salmonella enterica Serovar Typhimurium Type 1 Fimbriae Exhibit Increased Invasiveness for Mammalian Cells

Rebecca L. Wilson, Jessica Elthon, Steven Clegg, and Bradley D. Jones*

Department of Microbiology, University of Iowa, Iowa City, Iowa 52242

Received 18 February 2000/Returned for modification 5 April 2000/Accepted 26 April 2000

Salmonella enterica serovars Gallinarum and Pullorum are S. enterica biotypes that exhibit host specificity for poultry and aquatic birds and are not normally capable of causing disease in mammalian hosts. During their evolution toward host restriction serovars Gallinarum and Pullorum lost their ability to mediate mannose-sensitive hemagglutination (MSHA), a phenotype correlated with adherence to certain cell types. Because adherence is an essential requirement for invasion of cells by bacterial pathogens, we examined whether MHSA type 1 fimbriae would increase the ability of serovars Pullorum and Gallinarum to invade normally restrictive cells. Serovars Gallinarum and Pullorum expressing S. enterica serovar Typhimurium strain LT2 type 1 fimbriae exhibited a 10- to 20-fold increased ability to adhere to and a 20- to 60-fold increased invasion efficiency of the human epithelial HEp-2 cell line. Invasion was accompanied by extensive ruffling of the membranes of the HEp-2 cells. In a murine ligated ileal loop model, a 32% increase in the number of M-cell ruffles was seen when serovar Gallinarum expressed serovar Typhimurium type 1 fimbriae.

* Corresponding author. Mailing address: 3-330 Bowen Science Building, Department of Microbiology, University of Iowa, Iowa City, IA 52242. Phone: (319) 353-5457. Fax: (319) 335-9006. E-mail: bradley-jones{at}uiowa.edu.

Infection and Immunity, August 2000, p. 4782-4785, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Guo, A., Cao, S., Tu, L., Chen, P., Zhang, C., Jia, A., Yang, W., Liu, Z., Chen, H., Schifferli, D. M. (2009). FimH alleles direct preferential binding of Salmonella to distinct mammalian cells or to avian cells. Microbiology 155: 1623-1633 [Abstract] [Full Text]  
  • Jeong, J.-H., Song, M., Park, S.-I., Cho, K.-O., Rhee, J. H., Choy, H. E. (2008). Salmonella enterica Serovar Gallinarum Requires ppGpp for Internalization and Survival in Animal Cells. J. Bacteriol. 190: 6340-6350 [Abstract] [Full Text]  
  • Berndt, A., Wilhelm, A., Jugert, C., Pieper, J., Sachse, K., Methner, U. (2007). Chicken Cecum Immune Response to Salmonella enterica Serovars of Different Levels of Invasiveness. Infect. Immun. 75: 5993-6007 [Abstract] [Full Text]  
  • Orsi, R. H., Ripoll, D. R., Yeung, M., Nightingale, K. K., Wiedmann, M. (2007). Recombination and positive selection contribute to evolution of Listeria monocytogenes inlA. Microbiology 153: 2666-2678 [Abstract] [Full Text]  
  • Shah, D. H., Lee, M.-j., Park, J.-h., Lee, J.-h., Eo, S.-k., Kwon, J.-t., Chae, J.-s. (2005). Identification of Salmonella gallinarum virulence genes in a chicken infection model using PCR-based signature-tagged mutagenesis. Microbiology 151: 3957-3968 [Abstract] [Full Text]  
  • Kisiela, D., Sapeta, A., Kuczkowski, M., Stefaniak, T., Wieliczko, A., Ugorski, M. (2005). Characterization of FimH Adhesins Expressed by Salmonella enterica Serovar Gallinarum Biovars Gallinarum and Pullorum: Reconstitution of Mannose-Binding Properties by Single Amino Acid Substitution. Infect. Immun. 73: 6187-6190 [Abstract] [Full Text]  
  • Weening, E. H., Barker, J. D., Laarakker, M. C., Humphries, A. D., Tsolis, R. M., Baumler, A. J. (2005). The Salmonella enterica Serotype Typhimurium lpf, bcf, stb, stc, std, and sth Fimbrial Operons Are Required for Intestinal Persistence in Mice. Infect. Immun. 73: 3358-3366 [Abstract] [Full Text]  
  • Hess, P., Altenhofer, A., Khan, A. S., Daryab, N., Kim, K. S., Hacker, J., Oelschlaeger, T. A. (2004). A Salmonella fim Homologue in Citrobacter freundii Mediates Invasion In Vitro and Crossing of the Blood-Brain Barrier in the Rat Pup Model. Infect. Immun. 72: 5298-5307 [Abstract] [Full Text]  
  • Srinivasa Rao, P. S., Lim, T. M., Leung, K. Y. (2003). Functional Genomics Approach to the Identification of Virulence Genes Involved in Edwardsiella tarda Pathogenesis. Infect. Immun. 71: 1343-1351 [Abstract] [Full Text]  
  • Paulin, S. M., Watson, P. R., Benmore, A. R., Stevens, M. P., Jones, P. W., Villarreal-Ramos, B., Wallis, T. S. (2002). Analysis of Salmonella enterica Serotype-Host Specificity in Calves: Avirulence of S. enterica Serotype Gallinarum Correlates with Bacterial Dissemination from Mesenteric Lymph Nodes and Persistence In Vivo. Infect. Immun. 70: 6788-6797 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»