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Infection and Immunity, August 2000, p. 4782-4785, Vol. 68, No. 8
Department of Microbiology, University of
Iowa, Iowa City, Iowa 52242
Received 18 February 2000/Returned for modification 5 April
2000/Accepted 26 April 2000
Salmonella enterica serovars Gallinarum and Pullorum
are S. enterica biotypes that exhibit host specificity for
poultry and aquatic birds and are not normally capable of causing
disease in mammalian hosts. During their evolution toward host
restriction serovars Gallinarum and Pullorum lost their ability to
mediate mannose-sensitive hemagglutination (MSHA), a phenotype
correlated with adherence to certain cell types. Because adherence is
an essential requirement for invasion of cells by bacterial pathogens, we examined whether MHSA type 1 fimbriae would increase the ability of
serovars Pullorum and Gallinarum to invade normally restrictive cells.
Serovars Gallinarum and Pullorum expressing S. enterica serovar Typhimurium strain LT2 type 1 fimbriae exhibited a 10- to
20-fold increased ability to adhere to and a 20- to 60-fold increased
invasion efficiency of the human epithelial HEp-2 cell line. Invasion
was accompanied by extensive ruffling of the membranes of the HEp-2
cells. In a murine ligated ileal loop model, a 32% increase in the
number of M-cell ruffles was seen when serovar Gallinarum expressed
serovar Typhimurium type 1 fimbriae.
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Salmonella enterica Serovars Gallinarum and Pullorum
Expressing Salmonella enterica Serovar Typhimurium Type 1 Fimbriae Exhibit Increased Invasiveness for Mammalian
Cells
*
Corresponding author. Mailing address: 3-330 Bowen
Science Building, Department of Microbiology, University of Iowa, Iowa City, IA 52242. Phone: (319) 353-5457. Fax: (319) 335-9006. E-mail: bradley-jones{at}uiowa.edu.
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