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Infection and Immunity, August 2000, p. 4811-4814, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pseudomonas aeruginosa Exoenzyme S Induces Transcriptional Expression of Proinflammatory Cytokines and Chemokines

Slava Epelman,1 Tony F. Bruno,1 Graham G. Neely,2 Donald E. Woods,1 and Christopher H. Mody1,3,*

Department of Microbiology and Infectious Diseases,1 Department of Medical Sciences,2 and Department of Internal Medicine,3 University of Calgary, Calgary, Alberta T2N 4N1, Canada

Received 6 March 2000/Returned for modification 20 April 2000/Accepted 15 May 2000

Pseudomonas aeruginosa infection of cystic fibrosis patients causes lung damage that is substantially orchestrated by cytokines. In this study, multi-gene probe analysis was used to characterize the ability of the P. aeruginosa mitogen, exoenzyme S, to induce proinflammatory and immunoregulatory cytokines and chemokines. Exoenzyme S strongly induced transcription of proinflammatory cytokines and chemokines (tumor necrosis factor alpha, interleukin-1alpha [IL-1alpha ], IL-1beta , IL-6, IL-8, MIP-1alpha , MIP-1beta , MCP-1, RANTES, and I-309), modest transcription of immunoregulatory cytokines (IL-10 and IL-12p40), and weak transcription of Th1 cytokines (IL-2 and gamma interferon). The response occurred early and subsided without evolving over time. These data suggest that cells responding to exoenzyme S would rapidly express proinflammatory cytokines and chemokines that may contribute to pulmonary inflammation in cystic fibrosis.

* Corresponding author. Mailing address: Heritage Medical Research Building, Rm. 273, 3330 Hospital Dr., N.W., University of Calgary, Calgary, Alberta, Canada T2N-4N1. Phone: (403) 220-8479. Fax: (403) 270-2772. E-mail: cmody{at}ucalgary.ca.

Infection and Immunity, August 2000, p. 4811-4814, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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