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Infection and Immunity, September 2000, p. 4850-4855, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of a Shiga Toxin 2e-Converting Bacteriophage from an Escherichia coli Strain of Human Origin

Maite Muniesa, Jürgen Recktenwald, Martina Bielaszewska, Helge Karch, and Herbert Schmidt*

Institut für Hygiene und Mikrobiologie der Universität Würzburg 97080 Würzburg, Germany

Received 18 January 2000/Returned for modification 28 February 2000/Accepted 8 June 2000

An infectious Shiga toxin (Stx) 2e-converting bacteriophage (phi P27) was isolated from Stx2e-producing Escherichia coli ONT:H- isolate 2771/97 originating from a patient with diarrhea. The phage could be transduced to E. coli laboratory strain DH5alpha , and we could show that lysogens were able to produce biologically active toxin in a recA-dependent manner. By DNA sequence analysis of a 6,388-bp HindIII restriction fragment of phi P27, we demonstrated that the stx2e gene was located directly downstream of ileZ and argO tRNA genes. Although no analogue of an antiterminator Q encoding gene was present on this fragment, a lysis cassette comprising two holin genes which are related to the holin genes of Pseudomonas aeruginosa phage phi CTX and a gene homologous to the endolysin gene gp19 of phage PS3 were detected. The results of our study demonstrated for the first time that Stx2e can be encoded in the genome of an infectious bacteriophage.

* Corresponding author. Mailing address: Institut für Hygiene und Mikrobiologie der Universität Würzburg, Josef Schneider-Str. 2, 97080 Würzburg, Germany. Phone: 49-931-201-3905. Fax: 49-931-201-3445. E-mail: hschmidt{at}hygiene.uni-wuerzburg.de.

Infection and Immunity, September 2000, p. 4850-4855, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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