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Infection and Immunity, September 2000, p. 4948-4953, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Protective Immunity against Congenital Toxoplasmosis with Recombinant SAG1 Protein in a Guinea Pig Model

Michèle Haumont,1 Louis Delhaye,1 Lida Garcia,1 Margarita Jurado,1 Pasqualina Mazzu,1 Véronique Daminet,1 Vincent Verlant,2 Alex Bollen,1 Ralph Biemans,2 and Alain Jacquet1,*

Department of Applied Genetics, Institut de Biologie et de Médecine Moléculaires, Université Libre de Bruxelles, B-6041 Gosselies,1 and SmithKline Beecham Biologicals, B-1330 Rixensart,2 Belgium

Received 13 April 2000/Returned for modification 30 May 2000/Accepted 7 June 2000

Primary infection with Toxoplasma gondii during pregnancy can induce fetal pathology and abortion in both humans and animals. The present study describes the development of an experimental model of congenital toxoplasmosis in the guinea pig. In this animal model, we evaluated the protective effect of vaccination with a recombinant form of SAG1 against maternofetal transmission of tachyzoites. The presence of parasites in fetuses was determined by nested PCRs and by an in vivo readout after fetal brain homogenate injections in mice. The absence of parasites was demonstrated in 66 to 86% of fetuses derived from adult guinea pigs immunized with SAG1 and challenged with the mildly virulent T. gondii strain C56. In contrast, more than 80% of fetuses from mock-immunized guinea pigs were infected. The protection was not correlated with titers of antibody to SAG1. Our results indicated that this experimental model constitutes a relevant model for evaluation of vaccine candidates against congenital toxoplasmosis and that SAG1 elicits significant protection against maternofetal transmission.

* Corresponding author. Mailing address: Rue des Professeurs Jeener et Brachet, 12, B-6041 Gosselies, Belgium. Phone: 32 2 650 99 09. Fax: 32 2 650 99 00. E-mail: ajacquet{at}sga.ulb.ac.be.

Infection and Immunity, September 2000, p. 4948-4953, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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