This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hu, W.-G.
Right arrow Articles by Gu, X.-X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hu, W.-G.
Right arrow Articles by Gu, X.-X.

 Previous Article  |  Next Article 

Infection and Immunity, September 2000, p. 4980-4985, Vol. 68, No. 9
0019-9567/00/$04.00+0

Enhancement of Clearance of Bacteria from Murine Lungs by Immunization with Detoxified Lipooligosaccharide from Moraxella catarrhalis Conjugated to Proteins

Wei-Gang Hu, Jing Chen, James F. Battey, and Xin-Xing Gu*

Laboratory of Immunology, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Rockville, Maryland 20850

Received 22 February 2000/Returned for modification 1 May 2000/Accepted 9 June 2000

Moraxella catarrhalis strain 25238 detoxified lipooligosaccharide (dLOS)-protein conjugates induced a significant rise of bactericidal anti-LOS antibodies in animals. This study reports the effect of active or passive immunization with the conjugates or their antiserum on pulmonary clearance of M. catarrhalis in an aerosol challenge mouse model. Mice were injected subcutaneously with dLOS-tetanus toxoid (dLOS-TT), dLOS-high-molecular-weight proteins (dLOS-HMP) from nontypeable Haemophilus influenzae (NTHi), or nonconjugated materials in Ribi adjuvant and then challenged with M. catarrhalis strain 25238 or O35E or NTHi strain 12. Immunization with dLOS-TT or dLOS-HMP generated a significant rise of serum anti-LOS immunoglobulin G and 68% and 35 to 41% reductions of bacteria in lungs compared with the control (P < 0.01) following challenge with homologous strain 25238 and heterologous strain O35E, respectively. Serum anti-LOS antibody levels correlated with its bactericidal titers against M. catarrhalis and bacterial CFU in lungs. Additionally, immunization with dLOS-HMP generated a 54% reduction of NTHi strain 12 compared with the control (P < 0.01). Passive immunization with a rabbit antiserum against dLOS-TT conferred a significant reduction of strain 25238 CFU in lungs in a dose- and time-dependent pattern compared with preimmune serum-treated mice. Kinetic examination of lung tissue sections demonstrated that antiserum-treated mice initiated and offset inflammatory responses more rapidly than preimmune serum-treated mice. These data indicate that LOS antibodies (whether active or passive) play a major role in the enhancement of pulmonary clearance of different test strains of M. catarrhalis in mice. In addition, dLOS-HMP is a potential candidate for a bivalent vaccine against M. catarrhalis and NTHi infections.

* Corresponding author. Mailing address: NIDCD, NIH, 5 Research Court, 2A31, Rockville, MD 20850. Phone: (301) 402-2581. Fax: (301) 480-8019. E-mail: guxx{at}nidcd.nih.gov.

Infection and Immunity, September 2000, p. 4980-4985, Vol. 68, No. 9
0019-9567/00/$04.00+0


This article has been cited by other articles:

  • Schwingel, J. M., Edwards, K. J., Cox, A. D., Masoud, H., Richards, J. C., St. Michael, F., Tekwe, C. D., Sethi, S., Murphy, T. F., Campagnari, A. A. (2009). Use of Moraxella catarrhalis Lipooligosaccharide Mutants To Identify Specific Oligosaccharide Epitopes Recognized by Human Serum Antibodies. Infect. Immun. 77: 4548-4558 [Abstract] [Full Text]  
  • Yu, S., Xie, H., Datta, A., Naidu, N., Gu, X.-X. (2008). Galactose Residues on the Lipooligosaccharide of Moraxella catarrhalis 26404 Form the Epitope Recognized by the Bactericidal Antiserum from Conjugate Vaccination. Infect. Immun. 76: 4251-4258 [Abstract] [Full Text]  
  • Schwingel, J. M, Michael, F. S., Cox, A. D, Masoud, H., Richards, J. C, Campagnari, A. A (2008). A unique glycosyltransferase involved in the initial assembly of Moraxella catarrhalis lipooligosaccharides. Glycobiology 18: 447-455 [Abstract] [Full Text]  
  • Yu, S., Gu, X.-X. (2007). Biological and Immunological Characteristics of Lipooligosaccharide-Based Conjugate Vaccines for Serotype C Moraxella catarrhalis. Infect. Immun. 75: 2974-2980 [Abstract] [Full Text]  
  • Wieland, C. W., Florquin, S., Maris, N. A., Hoebe, K., Beutler, B., Takeda, K., Akira, S., van der Poll, T. (2005). The MyD88-Dependent, but Not the MyD88-Independent, Pathway of TLR4 Signaling Is Important in Clearing Nontypeable Haemophilus influenzae from the Mouse Lung. J. Immunol. 175: 6042-6049 [Abstract] [Full Text]  
  • Yu, S., Gu, X.-X. (2005). Synthesis and Characterization of Lipooligosaccharide-Based Conjugate Vaccines for Serotype B Moraxella catarrhalis. Infect. Immun. 73: 2790-2796 [Abstract] [Full Text]  
  • Edwards, K. J., Allen, S., Gibson, B. W., Campagnari, A. A. (2005). Characterization of a Cluster of Three Glycosyltransferase Enzymes Essential for Moraxella catarrhalis Lipooligosaccharide Assembly. J. Bacteriol. 187: 2939-2947 [Abstract] [Full Text]  
  • Furano, K., Luke, N. R., Howlett, A. J., Campagnari, A. A. (2005). Identification of a conserved Moraxella catarrhalis haemoglobin-utilization protein, MhuA. Microbiology 151: 1151-1158 [Abstract] [Full Text]  
  • Jiao, X., Hirano, T., Hou, Y., Gu, X.-X. (2002). Specific Immune Responses and Enhancement of Murine Pulmonary Clearance of Moraxella catarrhalis by Intranasal Immunization with a Detoxified Lipooligosaccharide Conjugate Vaccine. Infect. Immun. 70: 5982-5989 [Abstract] [Full Text]  
  • Verduin, C. M., Hol, C., Fleer, A., van Dijk, H., van Belkum, A. (2002). Moraxella catarrhalis: from Emerging to Established Pathogen. Clin. Microbiol. Rev. 15: 125-144 [Abstract] [Full Text]  
  • Horner, A. A., Datta, S. K., Takabayashi, K., Belyakov, I. M., Hayashi, T., Cinman, N., Nguyen, M.-D., Van Uden, J. H., Berzofsky, J. A., Richman, D. D., Raz, E. (2001). Immunostimulatory DNA-Based Vaccines Elicit Multifaceted Immune Responses Against HIV at Systemic and Mucosal Sites. J. Immunol. 167: 1584-1591 [Abstract] [Full Text]  
  • Hu, W.-G., Chen, J., McMichael, J. C., Gu, X.-X. (2001). Functional Characteristics of a Protective Monoclonal Antibody against Serotype A and C Lipooligosaccharides from Moraxella catarrhalis. Infect. Immun. 69: 1358-1363 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»