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Infection and Immunity, September 2000, p. 4980-4985, Vol. 68, No. 9
0019-9567/00/$04.00+0
Enhancement of Clearance of Bacteria from Murine
Lungs by Immunization with Detoxified Lipooligosaccharide from
Moraxella catarrhalis Conjugated to Proteins
Wei-Gang
Hu,
Jing
Chen,
James F.
Battey, and
Xin-Xing
Gu*
Laboratory of Immunology, National Institute
on Deafness and Other Communication Disorders, National Institutes
of Health, Rockville, Maryland 20850
Received 22 February 2000/Returned for modification 1 May
2000/Accepted 9 June 2000
Moraxella catarrhalis strain 25238 detoxified
lipooligosaccharide (dLOS)-protein conjugates induced a significant
rise of bactericidal anti-LOS antibodies in animals. This study reports the effect of active or passive immunization with the conjugates or
their antiserum on pulmonary clearance of M. catarrhalis in an aerosol challenge mouse model. Mice were injected subcutaneously with dLOS-tetanus toxoid (dLOS-TT), dLOS-high-molecular-weight proteins (dLOS-HMP) from nontypeable Haemophilus influenzae
(NTHi), or nonconjugated materials in Ribi adjuvant and then challenged with M. catarrhalis strain 25238 or O35E or NTHi strain 12. Immunization with dLOS-TT or dLOS-HMP generated a significant rise of
serum anti-LOS immunoglobulin G and 68% and 35 to 41% reductions of bacteria in lungs compared with the control (P < 0.01) following challenge with homologous strain 25238 and heterologous
strain O35E, respectively. Serum anti-LOS antibody levels correlated with its bactericidal titers against M. catarrhalis and
bacterial CFU in lungs. Additionally, immunization with dLOS-HMP
generated a 54% reduction of NTHi strain 12 compared with the control
(P < 0.01). Passive immunization with a rabbit
antiserum against dLOS-TT conferred a significant reduction of strain
25238 CFU in lungs in a dose- and time-dependent pattern compared with
preimmune serum-treated mice. Kinetic examination of lung tissue
sections demonstrated that antiserum-treated mice initiated and offset inflammatory responses more rapidly than preimmune serum-treated mice.
These data indicate that LOS antibodies (whether active or passive)
play a major role in the enhancement of pulmonary clearance of
different test strains of M. catarrhalis in mice. In
addition, dLOS-HMP is a potential candidate for a bivalent vaccine
against M. catarrhalis and NTHi infections.
*
Corresponding author. Mailing address: NIDCD, NIH, 5 Research Court, 2A31, Rockville, MD 20850. Phone: (301) 402-2581. Fax: (301) 480-8019. E-mail: guxx{at}nidcd.nih.gov.
Infection and Immunity, September 2000, p. 4980-4985, Vol. 68, No. 9
0019-9567/00/$04.00+0
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