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Infection and Immunity, September 2000, p. 5084-5089, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Differential Expression of Caveolin-1 in Lipopolysaccharide-Activated Murine Macrophages

Mei G. Lei1,* and David C. Morrison1,2,3

Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas,1 Office of Research Administration, St. Luke's Hospital, Kansas City, Missouri 64111,2 and Department of Basic Medical Science, University of Missouri at Kansas City, School of Medicine, Kansas City, Missouri 641083

Received 7 April 2000/Accepted 15 June 2000

Five reciprocal cycles of subtractive hybridization using cDNA generated from fibroblasts with normal lipopolysaccharide (LPS) responsiveness (lpsn) and from hyporesponsive (lpsd) fibroblasts have led to the finding that caveolin-1 is expressed at markedly higher levels of mRNA in lpsd than in lpsn fibroblasts. Caveolin-1 message can also be readily detected via reverse transcription-PCR in the RAW264.7 and J774.1 macrophage-like cell lines as well as in primary thioglycolate (TG)-elicited mouse peritoneal macrophages. In RAW264.7 cells, both caveolin-1 mRNA and protein levels are down-regulated by LPS. In TG-elicited C3HeB/FeJ peritoneal macrophages, in contrast, expression of both caveolin-1 protein and mRNA is up-regulated in vitro in response to LPS stimulation. The up-regulation of caveolin-1 protein expression in C3HeB/FeJ peritoneal macrophages can be demonstrated at concentrations as low as 1.0 pg of LPS/ml. However, LPS concentrations approximately 4 orders of magnitude higher (104 pg/ml) were required to stimulate the LPS-hyporesponsive C3H/HeJ mice peritoneal macrophages such that significant caveolin-1 protein up-regulation was detected. Caveolin-1, a principal component of plasmalemmal caveolae, has been reported as a potentially important regulator for signal transduction during cellular stimulation. The results described in this report suggest that caveolin-1 expression may be associated with LPS signaling/internalization.

* Corresponding author. Present address: Department of Basic Medical Science, University of Missouri at Kansas City, School of Medicine, Kansas City, MO 64108. Phone: (816) 235-6067. Fax: (816) 235-5527. E-mail: leim{at}umkc.edu.

Infection and Immunity, September 2000, p. 5084-5089, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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