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Infection and Immunity, September 2000, p. 5107-5113, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Helicobacter hepaticus Does Not Induce or Potentiate Colitis in Interleukin-10-Deficient Mice

Levinus A. Dieleman,1,* Annemarie Arends,1 Susan L. Tonkonogy,2 Marije S. Goerres,1 David W. Craft,3 Wetonia Grenther,2 Rance K. Sellon,2 Ed Balish,4 and R. Balfour Sartor1

Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill,1 and Department of Microbiology, University of North Carolina Hospitals,3 Chapel Hill, and College of Veterinary Medicine, North Carolina State University, Raleigh,2 North Carolina, and Departments of Surgery and Microbiology, University of Wisconsin, Madison, Wisconsin4

Received 15 October 1999/Returned for modification 22 December 1999/Accepted 6 June 2000

Helicobacter hepaticus has been reported to induce colitis, hepatitis, and hepatocellular carcinoma in several different murine models. The aim of this study was to determine if H. hepaticus will cause colitis in monoassociated mice lacking the interleukin-10 gene (IL-10-/- mice) and potentiate colitis in specific-pathogen-free (SPF) IL-10-/- mice. Germfree IL-10-/- mice on either a mixed (C57BL/6 × 129/Ola) or inbred (129/SvEv) genetic background were monoassociated with H. hepaticus ATCC 51448 by oral feeding and rectal enemas. In a second experiment, germfree IL-10-/- mice were colonized with stool from SPF mice that harbored or did not harbor endogenous H. hepaticus. After 7 to 9 weeks of colonization, weight loss and mortality were assessed, the colon was isolated for histology and IL-12 secretion, and mesenteric lymph node cells were assessed for T-cell activation markers. It was found that IL-10-/- mice monoassociated with H. hepaticus for up to 16 weeks showed almost no histologic colitis or increased IL-12 production. SPF IL-10-knockout mice had no significant difference in weight loss, mortality rate, histologic scores, colonic IL-12 secretion, or T-cell activation with or without H. hepaticus. We conclude that H. hepaticus does not induce or potentiate disease in our IL-10-/- mice and therefore is not required to induce colitis in genetically susceptible hosts.

* Corresponding author. Mailing address: Division of Digestive Diseases, Glaxo Bldg., Room 146, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7080. Phone: (919) 966-7886. Fax: (919) 966-6842. E-mail: ldiele{at}med.unc.edu.

Infection and Immunity, September 2000, p. 5107-5113, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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