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Infection and Immunity, September 2000, p. 5254-5260, Vol. 68, No. 9
Northwest Center for Medical Education,
Indiana University School of Medicine, Gary, Indiana
46408,1 and Departments of
Pathology2 and
Medicine,3 University of California San
Diego School of Medicine, the VA San Diego Healthcare System, San
Diego, California 92161
Received 27 March 2000/Returned for modification 29 May
2000/Accepted 20 June 2000
The purpose of this study was to identify the functional
significance of the binding of soluble CD14 (sCD14) to bacterial peptidoglycan (PGN) and to compare the structural requirements of sCD14
for the binding to PGN and lipopolysaccharide (LPS) and for
sCD14-mediated enhancement of PGN- and LPS-induced cell responses. sCD14 did not facilitate the responses of membrane CD14
(mCD14)-negative pre-B 70Z/3 cells to PGN, although it facilitated the
responses of these cells to LPS and although mCD14 facilitated the
responses of 70Z/3 cells to PGN. sCD14 enhanced mCD14-mediated cell
activation by both PGN and LPS, but only the responses to LPS, and not
to PGN, were enhanced by LPS-binding protein. Four 4- or
5-amino-acid-long sequences within the 65-amino-acid N-terminal region
of sCD14 were needed for binding to both PGN and LPS and for
enhancement of cell activation by both PGN and LPS. However, deletions
of individual sequences had different effects on the ability of sCD14 to bind to PGN and to LPS and on the ability to enhance the responses to PGN and to LPS. Thus, there are different structural requirements of
sCD14 for binding to PGN and to LPS and for the enhancement of PGN- and
LPS-induced cell activation.
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Soluble CD14 Enhances Membrane CD14-Mediated
Responses to Peptidoglycan: Structural Requirements Differ from
Those for Responses to Lipopolysaccharide
*
Corresponding author. Mailing address: Northwest Center
for Medical Education, Indiana University School of Medicine, 3400 Broadway, Gary, IN 46408. Phone: (219) 980-6535. Fax: (219) 980-6566. E-mail: rdziar{at}iunhaw1.iun.indiana.edu.
Infection and Immunity, September 2000, p. 5254-5260, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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