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Infection and Immunity, January 2001, p. 237-244, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.237-244.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Human T- and B-Cell Responses to Schistosoma mansoni Recombinant Glyceraldehyde 3-Phosphate Dehydrogenase Correlate with Resistance to Reinfection with S. mansoni or Schistosoma haematobium after Chemotherapy

Rashika El Ridi,1,2,* Charles B. Shoemaker,3 Faten Farouk,2 Naglaa H. El Sherif,4 and Ahmed Afifi1

Zoology Department, Faculty of Science, Cairo University, Cairo,1 and Biomedical Research Center for Infectious Diseases, The Egyptian Organization for Biological Products and Vaccines,2 and Department of Tropical Medicine, Theodore Bilharz Research Institute,4 Giza, Egypt, and Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts3

Received 22 February 2000/Returned for modification 13 April 2000/Accepted 6 October 2000

Recently we reported that human T- and B-cell recognition of a 42-kDa protein (p42) in soluble extracts of adult Schistosoma mansoni worms correlates with resistance to reinfection with S. mansoni or S. haematobium. Amino acid microsequencing of p42 revealed that it consists predominantly of schistosome glyceraldehyde 3-phosphate dehydrogenase (SG3PDH). We have expressed SG3PDH in Escherichia coli and purified the recombinant protein in a soluble and enzymatically active form. Recombinant SG3PDH (rSG3PDH) reacted with human monospecific antibodies to p42. Lymphoproliferation and production of interleukin-4 and gamma interferon (IFN-gamma ) after in vitro stimulation with rSG3PDH and serum isotype responses to rSG3PDH were examined in individuals with extremes of resistance and susceptibility to reinfection after treatment of previous S. mansoni or S. haematobium infection. Lymphoproliferation and IFN-gamma production in response to rSG3PDH and the presence of serum immunoglobulin G1 (IgG1), IgG3, and IgA antibodies to rSG3PDH generally characterized individuals who are resistant to reinfection after chemotherapy. The data indicate that T- and B-cell immune reactivity to rSG3PDH correlates with resistance to reinfection, confirming previous studies identifying SG3PDH as a target of protective immunity in humans, and suggest that SG3PDH should be investigated as a possible vaccine for human schistosomiasis.

* Corresponding author. Mailing address: Zoology Department, Faculty of Science, Cairo University, Cairo 12613, Egypt. Phone: (202) 5676-708. Fax: (202) 3602-988. E-mail:Rashika{at}FRCU.EUN.Eg.

Infection and Immunity, January 2001, p. 237-244, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.237-244.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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