This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Booth, M. C.
Right arrow Articles by Gilmore, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Booth, M. C.
Right arrow Articles by Gilmore, M. S.

 Previous Article  |  Next Article 

Infection and Immunity, January 2001, p. 345-352, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.345-352.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Clonal Associations among Staphylococcus aureus Isolates from Various Sites of Infection

Mary C. Booth,1,* Lisa M. Pence,1 Param Mahasreshti,1 Michelle C. Callegan,1 and Michael S. Gilmore1,2

Department of Ophthalmology,1 and Department of Microbiology and Immunology,2 The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma

Received 24 July 2000/Returned for modification 29 August 2000/Accepted 25 October 2000

A molecular epidemiological analysis was undertaken to identify lineages of Staphylococcus aureus that may be disproportionately associated with infection. Pulsed-field gel electrophoresis analysis of 405 S. aureus clinical isolates collected from various infection types and geographic locations was performed. Five distinct S. aureus lineages (SALs 1, 2, 4, 5, and 6) were identified, which accounted for 19.01, 9.14, 22.72, 10.12, and 4.69% of isolates, respectively. In addition, 85 lineages which occurred with frequencies of <2.5% were identified and were termed "sporadic." The most prevalent lineage was methicillin-resistant S. aureus (SAL 4). The second most prevalent lineage, SAL 1, was also isolated at a high frequency from the anterior nares of healthy volunteers, suggesting that its prevalence among clinical isolates may be a consequence of high carriage rates in humans. Gene-specific PCR was carried out to detect genes for a number of staphylococcal virulence traits. tst and cna were found to be significantly associated with prevalent lineages compared to sporadic lineages. When specific infection sites were examined, SAL 4 was significantly associated with respiratory tract infection, while SAL 2 was enriched among blood isolates. SAL 1 and SAL 5 were clonally related to SALs shown by others to be widespread in the clinical isolate population. We conclude from this study that at least five phylogenetic lineages of S. aureus are highly prevalent and widely distributed among clinical isolates. The traits that confer on these lineages a propensity to infect may suggest novel approaches to antistaphylococcal therapy.

* Corresponding author. Mailing address: C/o Michael S. Gilmore, University of Oklahoma Health Sciences Center, BRC 356, P.O. Box 26901, Oklahoma City, OK 73190. Phone: (405) 271-1084. Fax: (405) 271-8781. E-mail: mgilmore{at}aardvark.ou.edu.

Infection and Immunity, January 2001, p. 345-352, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.345-352.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Peerayeh, S. N., Azimian, A., Nejad, Q. B., Kashi, M. (2009). Prevalence of agr Specificity Groups Among Staphylococcus aureus Isolates From University Hospitals in Tehran. Lab Med 40: 27-29 [Abstract] [Full Text]  
  • O'Callaghan, R. J., McCormick, C. C., Caballero, A. R., Marquart, M. E., Gatlin, H. P., Fratkin, J. D. (2007). Age-Related Differences in Rabbits during Experimental Staphylococcus aureus Keratitis. IOVS 48: 5125-5131 [Abstract] [Full Text]  
  • Holtfreter, S., Grumann, D., Schmudde, M., Nguyen, H. T. T., Eichler, P., Strommenger, B., Kopron, K., Kolata, J., Giedrys-Kalemba, S., Steinmetz, I., Witte, W., Broker, B. M. (2007). Clonal Distribution of Superantigen Genes in Clinical Staphylococcus aureus Isolates. J. Clin. Microbiol. 45: 2669-2680 [Abstract] [Full Text]  
  • Petersdorf, S., Oberdorfer, K., Wendt, C. (2006). Longitudinal Study of the Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus at a University Hospital. J. Clin. Microbiol. 44: 4297-4302 [Abstract] [Full Text]  
  • Pullinger, G. D., Lopez-Benavides, M., Coffey, T. J., Williamson, J. H., Cursons, R. T., Summers, E., Lacy-Hulbert, J., Maiden, M. C., Leigh, J. A. (2006). Application of Streptococcus uberis Multilocus Sequence Typing: Analysis of the Population Structure Detected among Environmental and Bovine Isolates from New Zealand and the United Kingdom. Appl. Environ. Microbiol. 72: 1429-1436 [Abstract] [Full Text]  
  • Smith, E. M., Green, L. E., Medley, G. F., Bird, H. E., Fox, L. K., Schukken, Y. H., Kruze, J. V., Bradley, A. J., Zadoks, R. N., Dowson, C. G. (2005). Multilocus Sequence Typing of Intercontinental Bovine Staphylococcus aureus Isolates. J. Clin. Microbiol. 43: 4737-4743 [Abstract] [Full Text]  
  • Wright, J. S. III, Traber, K. E., Corrigan, R., Benson, S. A., Musser, J. M., Novick, R. P. (2005). The agr Radiation: an Early Event in the Evolution of Staphylococci. J. Bacteriol. 187: 5585-5594 [Abstract] [Full Text]  
  • Mork, T., Tollersrud, T., Kvitle, B., Jorgensen, H. J., Waage, S. (2005). Comparison of Staphylococcus aureus Genotypes Recovered from Cases of Bovine, Ovine, and Caprine Mastitis. J. Clin. Microbiol. 43: 3979-3984 [Abstract] [Full Text]  
  • Cassat, J. E., Dunman, P. M., McAleese, F., Murphy, E., Projan, S. J., Smeltzer, M. S. (2005). Comparative Genomics of Staphylococcus aureus Musculoskeletal Isolates. J. Bacteriol. 187: 576-592 [Abstract] [Full Text]  
  • Hu, D.-L., Cui, J.-C., Omoe, K., Sashinami, H., Yokomizo, Y., Shinagawa, K., Nakane, A. (2005). A Mutant of Staphylococcal Enterotoxin C Devoid of Bacterial Superantigenic Activity Elicits a Th2 Immune Response for Protection against Staphylococcus aureus Infection. Infect. Immun. 73: 174-180 [Abstract] [Full Text]  
  • van der Mee-Marquet, N., Domelier, A.-S., Girard, N., Quentin, R., the Bloodstream Infection Study Group of the Relai, (2004). Epidemiology and Typing of Staphylococcus aureus Strains Isolated from Bloodstream Infections. J. Clin. Microbiol. 42: 5650-5657 [Abstract] [Full Text]  
  • Trad, S., Allignet, J., Frangeul, L., Davi, M., Vergassola, M., Couve, E., Morvan, A., Kechrid, A., Buchrieser, C., Glaser, P., El Solh, N. (2004). DNA Macroarray for Identification and Typing of Staphylococcus aureus Isolates. J. Clin. Microbiol. 42: 2054-2064 [Abstract] [Full Text]  
  • Hall, A. E., Domanski, P. J., Patel, P. R., Vernachio, J. H., Syribeys, P. J., Gorovits, E. L., Johnson, M. A., Ross, J. M., Hutchins, J. T., Patti, J. M. (2003). Characterization of a Protective Monoclonal Antibody Recognizing Staphylococcus aureus MSCRAMM Protein Clumping Factor A. Infect. Immun. 71: 6864-6870 [Abstract] [Full Text]  
  • Vernachio, J., Bayer, A. S., Le, T., Chai, Y.-L., Prater, B., Schneider, A., Ames, B., Syribeys, P., Robbins, J., Patti, J. M. (2003). Anti-Clumping Factor A Immunoglobulin Reduces the Duration of Methicillin-Resistant Staphylococcus aureus Bacteremia in an Experimental Model of Infective Endocarditis. Antimicrob. Agents Chemother. 47: 3400-3406 [Abstract] [Full Text]  
  • Feil, E. J., Cooper, J. E., Grundmann, H., Robinson, D. A., Enright, M. C., Berendt, T., Peacock, S. J., Smith, J. M., Murphy, M., Spratt, B. G., Moore, C. E., Day, N. P. J. (2003). How Clonal Is Staphylococcus aureus?. J. Bacteriol. 185: 3307-3316 [Abstract] [Full Text]  
  • Blevins, J. S., Elasri, M. O., Allmendinger, S. D., Beenken, K. E., Skinner, R. A., Thomas, J. R., Smeltzer, M. S. (2003). Role of sarA in the Pathogenesis of Staphylococcus aureus Musculoskeletal Infection. Infect. Immun. 71: 516-523 [Abstract] [Full Text]  
  • Gilot, P., Lina, G., Cochard, T., Poutrel, B. (2002). Analysis of the Genetic Variability of Genes Encoding the RNA III-Activating Components Agr and TRAP in a Population of Staphylococcus aureus Strains Isolated from Cows with Mastitis. J. Clin. Microbiol. 40: 4060-4067 [Abstract] [Full Text]  
  • Peacock, S. J., de Silva, G. D. I., Justice, A., Cowland, A., Moore, C. E., Winearls, C. G., Day, N. P. J. (2002). Comparison of Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis as Tools for Typing Staphylococcus aureus Isolates in a Microepidemiological Setting. J. Clin. Microbiol. 40: 3764-3770 [Abstract] [Full Text]  
  • Karlsson, A., Arvidson, S. (2002). Variation in Extracellular Protease Production among Clinical Isolates of Staphylococcus aureus Due to Different Levels of Expression of the Protease Repressor sarA. Infect. Immun. 70: 4239-4246 [Abstract] [Full Text]  
  • Blevins, J. S., Beenken, K. E., Elasri, M. O., Hurlburt, B. K., Smeltzer, M. S. (2002). Strain-Dependent Differences in the Regulatory Roles of sarA and agr in Staphylococcus aureus. Infect. Immun. 70: 470-480 [Abstract] [Full Text]  
  • Jarraud, S., Mougel, C., Thioulouse, J., Lina, G., Meugnier, H., Forey, F., Nesme, X., Etienne, J., Vandenesch, F. (2002). Relationships between Staphylococcus aureus Genetic Background, Virulence Factors, agr Groups (Alleles), and Human Disease. Infect. Immun. 70: 631-641 [Abstract] [Full Text]  
  • Moore, P. C. L., Lindsay, J. A. (2001). Genetic Variation among Hospital Isolates of Methicillin-Sensitive Staphylococcus aureus: Evidence for Horizontal Transfer of Virulence Genes. J. Clin. Microbiol. 39: 2760-2767 [Abstract] [Full Text]  
  • Rice, K., Huesca, M., Vaz, D., McGavin, M. J. (2001). Variance in Fibronectin Binding and fnb Locus Polymorphisms in Staphylococcus aureus: Identification of Antigenic Variation in a Fibronectin Binding Protein Adhesin of the Epidemic CMRSA-1 Strain of Methicillin-Resistant S. aureus. Infect. Immun. 69: 3791-3799 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»