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Infection and Immunity, January 2001, p. 360-366, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.360-366.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Biochemical and Biological Properties of Staphylococcal Enterotoxin K

Paul M. Orwin,1 Donald Y. M. Leung,2 Heather L. Donahue,2 Richard P. Novick,3 and Patrick M. Schlievert1,*

Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota, 554551; Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 802062; and Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, New York 100163

Received 6 July 2000/Returned for modification 26 September 2000/Accepted 19 October 2000

Staphylococcus aureus is an important human pathogen which is implicated in a wide variety of diseases. Major determinants of the virulence of this organism include extracellular virulence factors. Staphylococcal enterotoxins (SEs) are important causative agents in staphylococcal toxic shock syndrome and food poisoning. Our study identified a novel enterotoxin, SEK, and examined its biochemical and biological properties. SEK had a molecular weight of 26,000 and an experimentally determined pI of between 7.0 and 7.5. SEK was secreted by clinical isolates of S. aureus. We demonstrated that SEK had many of the biological activities associated with the SEs, including superantigenicity, pyrogenicity, the ability to enhance the lethal effect of endotoxin, and lethality in a rabbit model when administered by subcutaneous miniosmotic pump. Recombinant SEK was shown to stimulate human CD4+ and CD8+ T cells in a Vbeta -specific manner; T-cells bearing Vbeta 5.1, 5.2, and 6.7 were significantly stimulated to proliferate.

* Corresponding author. Mailing address: Department of Microbiology, University of Minnesota Medical School, Box 196 Mayo Bldg., 420 Delaware St. SE, Minneapolis, MN 55455. Phone: (612) 624-9471. Fax: (612) 626-0623. E-mail: pats{at}lenti.med.umn.edu.

Infection and Immunity, January 2001, p. 360-366, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.360-366.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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