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Infection and Immunity, January 2001, p. 367-377, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.367-377.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Salmonella Pathogenicity Island 2 Influences Both Systemic Salmonellosis and Salmonella-Induced Enteritis in Calves

J. Bispham, B. N. Tripathi,dagger P. R. Watson, and T. S. Wallis*

Institute for Animal Health, Compton, Berkshire RG20 7NN, United Kingdom

Received 18 July 2000/Returned for modification 23 August 2000/Accepted 19 October 2000

We have used signature-tagged mutagenesis to identify mutants of the host-specific Salmonella enterica serotype Dublin which were avirulent in calves and/or BALB/c mice. A mutant with a transposon insertion in the sseD gene of Salmonella pathogenicity island 2 (SPI-2), which encodes a putative secreted effector protein, was identified. This mutant was recovered from the bovine host but not from the murine host following infection with a pool of serotype Dublin mutants. However, a pure inoculum of the sseD mutant was subsequently shown to be attenuated in calves following infection either by the intravenous route or by the oral route. The sseD mutant was fully invasive for bovine intestinal mucosa but was subsequently unable to proliferate to the same numbers as the parental strain in vivo. Both the sseD mutant and a second SPI-2 mutant, with a transposon insertion in the ssaT gene, induced significantly weaker secretory and inflammatory responses in bovine ligated ileal loops than did the parental strain. These results demonstrate that SPI-2 is required by serotype Dublin for the induction of both systemic and enteric salmonellosis in calves.

* Corresponding author. Mailing address: Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, United Kingdom. Phone: 44 (0)1635 577230. Fax: 44 (0)1635 577243. E-mail: timothy.wallis{at}bbsrc.ac.uk.

dagger Present address: Indian Veterinary Research Institute, Izatnagar-243122 (U.P.), India.

Infection and Immunity, January 2001, p. 367-377, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.367-377.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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