This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bispham, J.
Right arrow Articles by Wallis, T. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bispham, J.
Right arrow Articles by Wallis, T. S.

 Previous Article  |  Next Article 

Infection and Immunity, January 2001, p. 367-377, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.367-377.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Salmonella Pathogenicity Island 2 Influences Both Systemic Salmonellosis and Salmonella-Induced Enteritis in Calves

J. Bispham, B. N. Tripathi,dagger P. R. Watson, and T. S. Wallis*

Institute for Animal Health, Compton, Berkshire RG20 7NN, United Kingdom

Received 18 July 2000/Returned for modification 23 August 2000/Accepted 19 October 2000

We have used signature-tagged mutagenesis to identify mutants of the host-specific Salmonella enterica serotype Dublin which were avirulent in calves and/or BALB/c mice. A mutant with a transposon insertion in the sseD gene of Salmonella pathogenicity island 2 (SPI-2), which encodes a putative secreted effector protein, was identified. This mutant was recovered from the bovine host but not from the murine host following infection with a pool of serotype Dublin mutants. However, a pure inoculum of the sseD mutant was subsequently shown to be attenuated in calves following infection either by the intravenous route or by the oral route. The sseD mutant was fully invasive for bovine intestinal mucosa but was subsequently unable to proliferate to the same numbers as the parental strain in vivo. Both the sseD mutant and a second SPI-2 mutant, with a transposon insertion in the ssaT gene, induced significantly weaker secretory and inflammatory responses in bovine ligated ileal loops than did the parental strain. These results demonstrate that SPI-2 is required by serotype Dublin for the induction of both systemic and enteric salmonellosis in calves.

* Corresponding author. Mailing address: Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, United Kingdom. Phone: 44 (0)1635 577230. Fax: 44 (0)1635 577243. E-mail: timothy.wallis{at}bbsrc.ac.uk.

dagger Present address: Indian Veterinary Research Institute, Izatnagar-243122 (U.P.), India.

Infection and Immunity, January 2001, p. 367-377, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.367-377.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pullinger, G. D., Dziva, F., Charleston, B., Wallis, T. S., Stevens, M. P. (2008). Identification of Salmonella enterica Serovar Dublin-Specific Sequences by Subtractive Hybridization and Analysis of Their Role in Intestinal Colonization and Systemic Translocation in Cattle. Infect. Immun. 76: 5310-5321 [Abstract] [Full Text]  
  • Bustamante, V. H., Martinez, L. C., Santana, F. J., Knodler, L. A., Steele-Mortimer, O., Puente, J. L. (2008). HilD-mediated transcriptional cross-talk between SPI-1 and SPI-2. Proc. Natl. Acad. Sci. USA 105: 14591-14596 [Abstract] [Full Text]  
  • Hapfelmeier, S., Muller, A. J., Stecher, B., Kaiser, P., Barthel, M., Endt, K., Eberhard, M., Robbiani, R., Jacobi, C. A., Heikenwalder, M., Kirschning, C., Jung, S., Stallmach, T., Kremer, M., Hardt, W.-D. (2008). Microbe sampling by mucosal dendritic cells is a discrete, MyD88-independent stepin {Delta}invG S. Typhimurium colitis. JEM 205: 437-450 [Abstract] [Full Text]  
  • Pullinger, G. D., Paulin, S. M., Charleston, B., Watson, P. R., Bowen, A. J., Dziva, F., Morgan, E., Villarreal-Ramos, B., Wallis, T. S., Stevens, M. P. (2007). Systemic Translocation of Salmonella enterica Serovar Dublin in Cattle Occurs Predominantly via Efferent Lymphatics in a Cell-Free Niche and Requires Type III Secretion System 1 (T3SS-1) but Not T3SS-2. Infect. Immun. 75: 5191-5199 [Abstract] [Full Text]  
  • Carnell, S. C., Bowen, A., Morgan, E., Maskell, D. J., Wallis, T. S., Stevens, M. P. (2007). Role in virulence and protective efficacy in pigs of Salmonella enterica serovar Typhimurium secreted components identified by signature-tagged mutagenesis. Microbiology 153: 1940-1952 [Abstract] [Full Text]  
  • Morgan, E., Bowen, A. J., Carnell, S. C., Wallis, T. S., Stevens, M. P. (2007). SiiE Is Secreted by the Salmonella enterica Serovar Typhimurium Pathogenicity Island 4-Encoded Secretion System and Contributes to Intestinal Colonization in Cattle. Infect. Immun. 75: 1524-1533 [Abstract] [Full Text]  
  • Coombes, B. K., Coburn, B. A., Potter, A. A., Gomis, S., Mirakhur, K., Li, Y., Finlay, B. B. (2005). Analysis of the Contribution of Salmonella Pathogenicity Islands 1 and 2 to Enteric Disease Progression Using a Novel Bovine Ileal Loop Model and a Murine Model of Infectious Enterocolitis. Infect. Immun. 73: 7161-7169 [Abstract] [Full Text]  
  • Coburn, B., Li, Y., Owen, D., Vallance, B. A., Finlay, B. B. (2005). Salmonella enterica Serovar Typhimurium Pathogenicity Island 2 Is Necessary for Complete Virulence in a Mouse Model of Infectious Enterocolitis. Infect. Immun. 73: 3219-3227 [Abstract] [Full Text]  
  • Hapfelmeier, S., Stecher, B., Barthel, M., Kremer, M., Muller, A. J., Heikenwalder, M., Stallmach, T., Hensel, M., Pfeffer, K., Akira, S., Hardt, W.-D. (2005). The Salmonella Pathogenicity Island (SPI)-2 and SPI-1 Type III Secretion Systems Allow Salmonella Serovar typhimurium to Trigger Colitis via MyD88-Dependent and MyD88-Independent Mechanisms. J. Immunol. 174: 1675-1685 [Abstract] [Full Text]  
  • Stevens, M. P., Haque, A., Atkins, T., Hill, J., Wood, M. W., Easton, A., Nelson, M., Underwood-Fowler, C., Titball, R. W., Bancroft, G. J., Galyov, E. E. (2004). Attenuated virulence and protective efficacy of a Burkholderia pseudomallei bsa type III secretion mutant in murine models of melioidosis. Microbiology 150: 2669-2676 [Abstract] [Full Text]  
  • Kim, C. C., Falkow, S. (2004). Delineation of Upstream Signaling Events in the Salmonella Pathogenicity Island 2 Transcriptional Activation Pathway. J. Bacteriol. 186: 4694-4704 [Abstract] [Full Text]  
  • Hapfelmeier, S., Ehrbar, K., Stecher, B., Barthel, M., Kremer, M., Hardt, W.-D. (2004). Role of the Salmonella Pathogenicity Island 1 Effector Proteins SipA, SopB, SopE, and SopE2 in Salmonella enterica Subspecies 1 Serovar Typhimurium Colitis in Streptomycin-Pretreated Mice. Infect. Immun. 72: 795-809 [Abstract] [Full Text]  
  • Zhang, S., Kingsley, R. A., Santos, R. L., Andrews-Polymenis, H., Raffatellu, M., Figueiredo, J., Nunes, J., Tsolis, R. M., Adams, L. G., Baumler, A. J. (2003). Molecular Pathogenesis of Salmonella enterica Serotype Typhimurium-Induced Diarrhea. Infect. Immun. 71: 1-12 [Full Text]  
  • Struve, C., Forestier, C., Krogfelt, K. A. (2003). Application of a novel multi-screening signature-tagged mutagenesis assay for identification of Klebsiella pneumoniae genes essential in colonization and infection. Microbiology 149: 167-176 [Abstract] [Full Text]  
  • Paulin, S. M., Watson, P. R., Benmore, A. R., Stevens, M. P., Jones, P. W., Villarreal-Ramos, B., Wallis, T. S. (2002). Analysis of Salmonella enterica Serotype-Host Specificity in Calves: Avirulence of S. enterica Serotype Gallinarum Correlates with Bacterial Dissemination from Mesenteric Lymph Nodes and Persistence In Vivo. Infect. Immun. 70: 6788-6797 [Abstract] [Full Text]  
  • Jones, M. A., Wigley, P., Page, K. L., Hulme, S. D., Barrow, P. A. (2001). Salmonella enterica Serovar Gallinarum Requires the Salmonella Pathogenicity Island 2 Type III Secretion System but Not the Salmonella Pathogenicity Island 1 Type III Secretion System for Virulence in Chickens. Infect. Immun. 69: 5471-5476 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»