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Infection and Immunity, January 2001, p. 367-377, Vol. 69, No. 1
Institute for Animal Health, Compton,
Berkshire RG20 7NN, United Kingdom
Received 18 July 2000/Returned for modification 23 August
2000/Accepted 19 October 2000
We have used signature-tagged mutagenesis to identify mutants of
the host-specific Salmonella enterica serotype Dublin
which were avirulent in calves and/or BALB/c mice. A mutant with a
transposon insertion in the sseD gene of
Salmonella pathogenicity island 2 (SPI-2), which encodes
a putative secreted effector protein, was identified. This mutant was
recovered from the bovine host but not from the murine host following
infection with a pool of serotype Dublin mutants. However, a
pure inoculum of the sseD mutant was subsequently shown
to be attenuated in calves following infection either by the
intravenous route or by the oral route. The sseD mutant
was fully invasive for bovine intestinal mucosa but was subsequently
unable to proliferate to the same numbers as the parental strain in
vivo. Both the sseD mutant and a second SPI-2 mutant,
with a transposon insertion in the ssaT gene, induced significantly weaker secretory and inflammatory responses in bovine ligated ileal loops than did the parental strain. These results demonstrate that SPI-2 is required by serotype Dublin for the induction
of both systemic and enteric salmonellosis in calves.
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.1.367-377.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Salmonella Pathogenicity Island 2 Influences Both Systemic Salmonellosis and
Salmonella-Induced Enteritis in Calves

*
Corresponding author. Mailing address: Institute for
Animal Health, Compton, Newbury, Berkshire RG20 7NN, United Kingdom. Phone: 44 (0)1635 577230. Fax: 44 (0)1635 577243. E-mail:
timothy.wallis{at}bbsrc.ac.uk.
Present address: Indian Veterinary Research Institute,
Izatnagar-243122 (U.P.), India.
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