This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chai, W.
Right arrow Articles by Lawson, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chai, W.
Right arrow Articles by Lawson, A. M.

 Previous Article  |  Next Article 

Infection and Immunity, January 2001, p. 420-425, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.420-425.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Inhibition of Adhesion of Plasmodium falciparum-Infected Erythrocytes by Structurally Defined Hyaluronic Acid Dodecasaccharides

Wengang Chai,1 James G. Beeson,2 Heide Kogelberg,1 Graham V. Brown,3 and Alexander M. Lawson1,*

MRC Glycosciences Laboratory, Imperial College School of Medicine, Northwick Park Hospital, Harrow, Middlesex HA1 3UJ, United Kingdom,1 and Division of Infection and Immunity, The Walter and Eliza Hall Institute of Medical Research,2 and Department of Medicine, The University of Melbourne,3 Parkville, Victoria 3050, Australia

Received 24 July 2000/Returned for modification 16 September 2000/Accepted 11 October 2000

We recently reported that Plasmodium falciparum-infected erythrocytes (IRBCs) can adhere to hyaluronic acid (HA), which appears to be a receptor, in addition to chondroitin sulfate A (CSA), for parasite sequestration in the placenta. Further investigations of the nature and specificity of this interaction indicate that HA oligosaccharide fragments competitively inhibit parasite adhesion to immobilized purified HA in a size-dependent manner, with dodecasaccharides being the minimum size for maximum inhibition. Rigorously purified and structurally defined HA dodecasaccharides, free of contamination by CSA or other glycosaminoglycans, effectively inhibited IRBC adhesion to HA but not CSA, providing compelling evidence of a specific interaction between IRBCs and HA.

* Corresponding author. Mailing address: Glycosciences Laboratory, Imperial College School of Medicine, Northwick Park Hospital, Watford Rd., Harrow, Middlesex HA1 3UJ, United Kingdom. Phone: 44-20-8869 3250. Fax: 44-20-8869 3253. E-mail: a.m.lawson{at}ic.ac.uk.

Infection and Immunity, January 2001, p. 420-425, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.420-425.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sotimehin, S. A., Runsewe-Abiodun, T. I., Oladapo, O. T., Njokanma, O. F., Olanrewaju, D. M. (2008). Possible Risk Factors for Congenital Malaria at a Tertiary Care Hospital in Sagamu, Ogun State, South-West Nigeria. J Trop Pediatr 54: 313-320 [Abstract] [Full Text]  
  • Beeson, J. G., Andrews, K. T., Boyle, M., Duffy, M. F., Choong, E. K., Byrne, T. J., Chesson, J. M., Lawson, A. M., Chai, W. (2007). Structural Basis for Binding of Plasmodium falciparum Erythrocyte Membrane Protein 1 to Chondroitin Sulfate and Placental Tissue and the Influence of Protein Polymorphisms on Binding Specificity. J. Biol. Chem. 282: 22426-22436 [Abstract] [Full Text]  
  • Muthusamy, A., Achur, R. N., Valiyaveettil, M., Botti, J. J., Taylor, D. W., Leke, R. F., Gowda, D. C. (2007). Chondroitin Sulfate Proteoglycan but Not Hyaluronic Acid Is the Receptor for the Adherence of Plasmodium falciparum-Infected Erythrocytes in Human Placenta, and Infected Red Blood Cell Adherence Up-Regulates the Receptor Expression. Am. J. Pathol. 170: 1989-2000 [Abstract] [Full Text]  
  • Andrews, K. T., Klatt, N., Adams, Y., Mischnick, P., Schwartz-Albiez, R. (2005). Inhibition of Chondroitin-4-Sulfate-Specific Adhesion of Plasmodium falciparum-Infected Erythrocytes by Sulfated Polysaccharides. Infect. Immun. 73: 4288-4294 [Abstract] [Full Text]  
  • Cot, M, Deloron, P (2003). Malaria prevention strategies: Pregnancy-Associated Malaria (PAM). Br Med Bull 67: 137-148 [Abstract] [Full Text]  
  • Volpi, N. (2003). Milligram-scale preparation and purification of oligosaccharides of defined length possessing the structure of chondroitin from defructosylated capsular polysaccharide K4. Glycobiology 13: 635-640 [Abstract] [Full Text]  
  • Chai, W., Beeson, J. G., Lawson, A. M. (2002). The Structural Motif in Chondroitin Sulfate for Adhesion of Plasmodium falciparum-infected Erythrocytes Comprises Disaccharide Units of 4-O-Sulfated and Non-sulfated N-Acetylgalactosamine Linked to Glucuronic Acid. J. Biol. Chem. 277: 22438-22446 [Abstract] [Full Text]  
  • Mahoney, D. J., Aplin, R. T., Calabro, A., Hascall, V. C., Day, A. J. (2001). Novel methods for the preparation and characterization of hyaluronan oligosaccharides of defined length. Glycobiology 11: 1025-1033 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»