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Infection and Immunity, January 2001, p. 494-500, Vol. 69, No. 1
Department of Microbiology and Immunology,
Seoul National University College of Medicine, and Institute of Endemic
Disease, Seoul National University Medical Research Center, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Republic of Korea
Received 10 July 2000/Returned for modification 14 September
2000/Accepted 15 October 2000
The host cell microfilaments and microtubules (MTs) are known to
play a critical role in the life cycles of several pathogenic intracellular microbes by providing for successful invasion and promoting movement of the pathogen once inside the host cell cytoplasm. Orientia tsutsugamushi, an obligate intracellular
bacterium, enters host cells by induced phagocytosis, escapes to the
cytosol, and then replicates in the cytosol. ECV304 cells infected with
O. tsutsugamushi revealed the colocalization of the MT
organizing center (MTOC) and cytosolic orientiae by indirect
immunofluorescence assay. Using immunofluorescence microscopy in the
presence and absence of MT-depolymerizing agents (colchicine and
nocodazole), it was shown that the cytosolic oriential movement was
mediated by MTs. By transfection study (overexpression of dynamitin
[also called p50], which is known to associate with dynein-dependent movement), the movement of O. tsutsugamushi to the MTOC was
also mediated by dynein, the minus-end-directed MT-related motor.
Although the significance of this movement in the life cycle of
O. tsutsugamushi was not proven, we propose that the
cytosolic O. tsutsugamushi bacteria use MTs and dyneins to
propel themselves from the cell periphery to the MTOC.
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.1.494-500.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Microtubule- and Dynein-Mediated Movement of
Orientia tsutsugamushi to the Microtubule Organizing
Center
*
Corresponding author. Mailing address: Department of
Microbiology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Republic of Korea. Phone:
82-2-740-8305. Fax: 82-2-743-0881. E-mail:
myung{at}plaza.snu.ac.kr.
Infection and Immunity, January 2001, p. 494-500, Vol. 69, No. 1
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.1.494-500.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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