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Infection and Immunity, January 2001, p. 52-57, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.52-57.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Enterotoxigenic Escherichia coli TibA Glycoprotein Adheres to Human Intestine Epithelial Cells

Christoph Lindenthaldagger and Eric A. Elsinghorst*

Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045-2106

Received 14 July 2000/Accepted 20 September 2000

Enterotoxigenic Escherichia coli (ETEC) is capable of invading epithelial cell lines derived from the human ileum and colon. Two separate invasion loci (tia and tib) that direct noninvasive E. coli strains to adhere to and invade cultured human intestine epithelial cells have previously been isolated from the classical ETEC strain H10407. The tib locus directs the synthesis of TibA, a 104-kDa outer membrane glycoprotein. Synthesis of TibA is directly correlated with the adherence and invasion phenotypes of the tib locus, suggesting that this protein is an adhesin and invasin. Here we report the purification of TibA and characterization of its biological activity. TibA was purified by continuous-elution preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Purified TibA was biotin labeled and then shown to bind to HCT8 human ileocecal epithelial cells in a specific and saturable manner. Unlabeled TibA competed with biotin-labeled TibA, suggesting the presence of a specific TibA receptor in HCT8 cells. These results show that TibA acts as an adhesin. Polyclonal anti-TibA antiserum inhibited invasion of ETEC strain H10407 and of recombinant E. coli bearing tib locus clones, suggesting that TibA also acts as an invasin. The ability of TibA to direct epithelial cell adhesion suggests a role for this protein in ETEC pathogenesis.

* Corresponding author. Mailing address: University of Kansas, Department of Molecular Biosciences, 7049 Haworth Hall, Lawrence, KS 66045-2106. Phone: (785) 864-4299. Fax: (785) 864-5294. E-mail: elsingh{at}ukans.edu.

dagger Present address: Institut fuer Tropenmedizin Universitaet Tuebingen, D-72074 Tuebingen, Germany.

Infection and Immunity, January 2001, p. 52-57, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.52-57.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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