This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Beckert, S.
Right arrow Articles by Podbielski, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beckert, S.
Right arrow Articles by Podbielski, A.

 Previous Article  |  Next Article 

Infection and Immunity, January 2001, p. 534-537, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.534-537.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Group A Streptococcal rofA Gene Is Involved in the Control of Several Virulence Genes and Eukaryotic Cell Attachment and Internalization

Susanne Beckert, Bernd Kreikemeyer, and Andreas Podbielski*

Department of Medical Microbiology, University Hospital Ulm, D-89081 Ulm, Germany

Received 24 April 2000/Returned for modification 11 August 2000/Accepted 2 October 2000

The serotype M6 group A streptococcal RofA regulator was previously shown to exert a direct positive control of protein F1 expression and, concomitantly, fibronectin binding. Using a serotype M6 rofA mutant, we demonstrate here that this regulator has a potentially indirect negative influence on the expression of the mga, emm6, pel-sagA, and speA virulence genes. Additionally, the rofA mutant exhibited reduced eukaryotic cell internalization rates in combination with decreased host cell viability.

* Corresponding author. Mailing address: Department of Medical Microbiology and Hygiene, University Hospital Rostock, Schillingallee 70, D-18055 Rostock, Germany. Phone: 49-731-502-4615. Fax: 49-731-502-4619. E-mail: andreas.podbielski{at}med.uni-rostock.de.

Infection and Immunity, January 2001, p. 534-537, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.534-537.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nobbs, A. H., Lamont, R. J., Jenkinson, H. F. (2009). Streptococcus Adherence and Colonization. Microbiol. Mol. Biol. Rev. 73: 407-450 [Abstract] [Full Text]  
  • Leday, T. V., Gold, K. M., Kinkel, T. L., Roberts, S. A., Scott, J. R., McIver, K. S. (2008). TrxR, a New CovR-Repressed Response Regulator That Activates the Mga Virulence Regulon in Group A Streptococcus. Infect. Immun. 76: 4659-4668 [Abstract] [Full Text]  
  • Rosch, J. W., Mann, B., Thornton, J., Sublett, J., Tuomanen, E. (2008). Convergence of Regulatory Networks on the Pilus Locus of Streptococcus pneumoniae. Infect. Immun. 76: 3187-3196 [Abstract] [Full Text]  
  • Kreikemeyer, B., Nakata, M., Koller, T., Hildisch, H., Kourakos, V., Standar, K., Kawabata, S., Glocker, M. O., Podbielski, A. (2007). The Streptococcus pyogenes Serotype M49 Nra-Ralp3 Transcriptional Regulatory Network and Its Control of Virulence Factor Expression from the Novel eno ralp3 epf sagA Pathogenicity Region. Infect. Immun. 75: 5698-5710 [Abstract] [Full Text]  
  • Oliver, M. A., Garcia-Rey, C., Bosch, R., Alberti, S. (2007). Evaluation of the ability of erythromycin-resistant and -susceptible pharyngeal group A Streptococcus isolates from Spain to enter and persist in human keratinocytes. J Med Microbiol 56: 1485-1489 [Abstract] [Full Text]  
  • Kratovac, Z., Manoharan, A., Luo, F., Lizano, S., Bessen, D. E. (2007). Population Genetics and Linkage Analysis of Loci within the FCT Region of Streptococcus pyogenes. J. Bacteriol. 189: 1299-1310 [Abstract] [Full Text]  
  • Kwinn, L. A., Khosravi, A., Aziz, R. K., Timmer, A. M., Doran, K. S., Kotb, M., Nizet, V. (2007). Genetic Characterization and Virulence Role of the RALP3/LSA Locus Upstream of the Streptolysin S Operon in Invasive M1T1 Group A Streptococcus. J. Bacteriol. 189: 1322-1329 [Abstract] [Full Text]  
  • Lembke, C., Podbielski, A., Hidalgo-Grass, C., Jonas, L., Hanski, E., Kreikemeyer, B. (2006). Characterization of Biofilm Formation by Clinically Relevant Serotypes of Group A Streptococci. Appl. Environ. Microbiol. 72: 2864-2875 [Abstract] [Full Text]  
  • Shelburne, S. A. III, Sumby, P., Sitkiewicz, I., Granville, C., DeLeo, F. R., Musser, J. M. (2005). Central role of a bacterial two-component gene regulatory system of previously unknown function in pathogen persistence in human saliva. Proc. Natl. Acad. Sci. USA 102: 16037-16042 [Abstract] [Full Text]  
  • Chaussee, M. A., Callegari, E. A., Chaussee, M. S. (2004). Rgg Regulates Growth Phase-Dependent Expression of Proteins Associated with Secondary Metabolism and Stress in Streptococcus pyogenes. J. Bacteriol. 186: 7091-7099 [Abstract] [Full Text]  
  • Towers, R. J., Gal, D., McMillan, D., Sriprakash, K. S., Currie, B. J., Walker, M. J., Chhatwal, G. S., Fagan, P. K. (2004). Fibronectin-Binding Protein Gene Recombination and Horizontal Transfer between Group A and G Streptococci. J. Clin. Microbiol. 42: 5357-5361 [Abstract] [Full Text]  
  • Gutekunst, H., Eikmanns, B. J., Reinscheid, D. J. (2003). Analysis of RogB-Controlled Virulence Mechanisms and Gene Expression in Streptococcus agalactiae. Infect. Immun. 71: 5056-5064 [Abstract] [Full Text]  
  • Graham, M. R., Smoot, L. M., Migliaccio, C. A. L., Virtaneva, K., Sturdevant, D. E., Porcella, S. F., Federle, M. J., Adams, G. J., Scott, J. R., Musser, J. M. (2002). Virulence control in group A Streptococcus by a two-component gene regulatory system: Global expression profiling and in vivo infection modeling. Proc. Natl. Acad. Sci. USA 99: 13855-13860 [Abstract] [Full Text]  
  • Fuller, J. D., Camus, A. C., Duncan, C. L., Nizet, V., Bast, D. J., Thune, R. L., Low, D. E., de Azavedo, J. C. S. (2002). Identification of a Streptolysin S-Associated Gene Cluster and Its Role in the Pathogenesis of Streptococcus iniae Disease. Infect. Immun. 70: 5730-5739 [Abstract] [Full Text]  
  • Spellerberg, B., Rozdzinski, E., Martin, S., Weber-Heynemann, J., Lutticken, R. (2002). rgf Encodes a Novel Two-Component Signal Transduction System of Streptococcus agalactiae. Infect. Immun. 70: 2434-2440 [Abstract] [Full Text]  
  • Kreikemeyer, B., Beckert, S., Braun-Kiewnick, A., Podbielski, A. (2002). Group A streptococcal RofA-type global regulators exhibit a strain-specific genomic presence and regulation pattern. Microbiology 148: 1501-1511 [Abstract] [Full Text]  
  • Chaussee, M. S., Sylva, G. L., Sturdevant, D. E., Smoot, L. M., Graham, M. R., Watson, R. O., Musser, J. M. (2002). Rgg Influences the Expression of Multiple Regulatory Loci To Coregulate Virulence Factor Expression in Streptococcus pyogenes. Infect. Immun. 70: 762-770 [Abstract] [Full Text]  
  • Biswas, I., Germon, P., McDade, K., Scott, J. R. (2001). Generation and Surface Localization of Intact M Protein in Streptococcus pyogenes Are Dependent on sagA. Infect. Immun. 69: 7029-7038 [Abstract] [Full Text]  
  • Smoot, L. M., Smoot, J. C., Graham, M. R., Somerville, G. A., Sturdevant, D. E., Migliaccio, C. A. L., Sylva, G. L., Musser, J. M. (2001). Global differential gene expression in response to growth temperature alteration in group A Streptococcus. Proc. Natl. Acad. Sci. USA 10.1073/pnas.191267598v1 [Abstract] [Full Text]  
  • Smoot, L. M., Smoot, J. C., Graham, M. R., Somerville, G. A., Sturdevant, D. E., Migliaccio, C. A. L., Sylva, G. L., Musser, J. M. (2001). Global differential gene expression in response to growth temperature alteration in group A Streptococcus. Proc. Natl. Acad. Sci. USA 98: 10416-10421 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»