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Infection and Immunity, January 2001, p. 538-542, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.538-542.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Expression of ExsA in trans Confers Type III Secretion System-Dependent Cytotoxicity on Noncytotoxic Pseudomonas aeruginosa Cystic Fibrosis Isolates

Denis Dacheux, Ina Attree, and Bertrand Toussaint*

Département de Biologie Moléculaire et Structurale, BBSI, UMR 5092 CNRS, CEA Grenoble, 38054 Grenoble Cedex 09, France

Received 1 June 2000/Returned for modification 18 August 2000/Accepted 4 October 2000

Twelve Pseudomonas aeruginosa cystic fibrosis isolates that are not able to exert a type III secretion system (TTSS)-dependent cytotoxicity towards phagocytes have been further studied. The strains, although possessing TTSS genes and exsA, which encodes a positive regulator of the TTSS regulon, showed no transcriptional activation of the exsCBA regulatory operon. The expression of exsA in trans restored the in vitro secretion of TTSS proteins and ex vivo cytotoxicity.

* Corresponding author. Mailing address: DBMS/BBSI, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble cedex 09, France. Phone: 33.4.76.88.34.83. Fax: 33.4.76.88.51.85. E-mail: btoussaint{at}cea.fr.

Infection and Immunity, January 2001, p. 538-542, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.538-542.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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