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Infection and Immunity, January 2001, p. 538-542, Vol. 69, No. 1
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.1.538-542.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Expression of ExsA in trans Confers Type III
Secretion System-Dependent Cytotoxicity on Noncytotoxic
Pseudomonas aeruginosa Cystic Fibrosis
Isolates
Denis
Dacheux,
Ina
Attree, and
Bertrand
Toussaint*
Département de Biologie
Moléculaire et Structurale, BBSI, UMR 5092 CNRS, CEA
Grenoble, 38054 Grenoble Cedex 09, France
Received 1 June 2000/Returned for modification 18 August
2000/Accepted 4 October 2000
Twelve Pseudomonas aeruginosa cystic fibrosis isolates
that are not able to exert a type III secretion system (TTSS)-dependent cytotoxicity towards phagocytes have been further studied. The strains,
although possessing TTSS genes and exsA, which encodes a
positive regulator of the TTSS regulon, showed no transcriptional activation of the exsCBA regulatory operon. The expression
of exsA in trans restored the in vitro secretion of TTSS
proteins and ex vivo cytotoxicity.
*
Corresponding author. Mailing address: DBMS/BBSI, CEA
Grenoble, 17 rue des Martyrs, 38054 Grenoble cedex 09, France.
Phone: 33.4.76.88.34.83. Fax: 33.4.76.88.51.85. E-mail:
btoussaint{at}cea.fr.
Infection and Immunity, January 2001, p. 538-542, Vol. 69, No. 1
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.1.538-542.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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