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Infection and Immunity, January 2001, p. 547-550, Vol. 69, No. 1
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.1.547-550.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Aromatic Compound-Dependent Brucella
suis Is Attenuated in Both Cultured Cells and Mouse
Models
Vincent
Foulongne,1
Karl
Walravens,2
Giséle
Bourg,1
Maria Laura
Boschiroli,1
Jacques
Godfroid,2
Michel
Ramuz,1 and
David
O'Callaghan1,*
INSERM U431, Faculté de Médecine,
30900 Nîmes, France,1 and
Veterinary and Agrochemical Research Center, B-1180
Brussels, Belgium2
Received 5 July 2000/Returned for modification 29 August
2000/Accepted 12 October 2000
The aroC gene of the facultative intracellular pathogen
Brucella suis was cloned and sequenced. The cloned
aroC gene complements Escherichia coli and
Salmonella enterica serovar Typhimurium aroC mutants. A B. suis aroC mutant was found to be unable to
grow in a defined medium without aromatic compounds. The mutant was highly attenuated in tissue culture (THP1 macrophages and HeLa cells)
and murine virulence models.
*
Corresponding author. Mailing address: INSERM U431,
Faculté de Médecine, Ave. Kennedy, 30900 Nîmes,
France. Phone: (33) 4 66 23 48 99. Fax: (33) 4 66 23 49 28. E-mail:
docallaghan{at}zeus.sc.univ-montp1.fr.
Infection and Immunity, January 2001, p. 547-550, Vol. 69, No. 1
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.1.547-550.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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