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Infection and Immunity, January 2001, p. 547-550, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.547-550.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Aromatic Compound-Dependent Brucella suis Is Attenuated in Both Cultured Cells and Mouse Models

Vincent Foulongne,1 Karl Walravens,2 Giséle Bourg,1 Maria Laura Boschiroli,1 Jacques Godfroid,2 Michel Ramuz,1 and David O'Callaghan1,*

INSERM U431, Faculté de Médecine, 30900 Nîmes, France,1 and Veterinary and Agrochemical Research Center, B-1180 Brussels, Belgium2

Received 5 July 2000/Returned for modification 29 August 2000/Accepted 12 October 2000

The aroC gene of the facultative intracellular pathogen Brucella suis was cloned and sequenced. The cloned aroC gene complements Escherichia coli and Salmonella enterica serovar Typhimurium aroC mutants. A B. suis aroC mutant was found to be unable to grow in a defined medium without aromatic compounds. The mutant was highly attenuated in tissue culture (THP1 macrophages and HeLa cells) and murine virulence models.

* Corresponding author. Mailing address: INSERM U431, Faculté de Médecine, Ave. Kennedy, 30900 Nîmes, France. Phone: (33) 4 66 23 48 99. Fax: (33) 4 66 23 49 28. E-mail: docallaghan{at}zeus.sc.univ-montp1.fr.

Infection and Immunity, January 2001, p. 547-550, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.547-550.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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