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Infection and Immunity, January 2001, p. 607-612, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.607-612.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Protective Epitopes by Sequencing of the Major Outer Membrane Protein Gene of a Variant Strain of Chlamydia psittaci Serotype 1 (Chlamydophila abortus)

Evangelia Vretou,^1,* Evgenia Psarrou,¹ Maria Kaisar,¹ Isabella Vlisidou,¹ Viviane Salti-Montesanto,¹ and David Longbottom²

Department of Microbiology, Hellenic Pasteur Institute, Athens 115 21, Greece,¹ and Moredun Research Institute, International Research Centre, Pentlands Science Park, Penicuik, Midlothian EH26 OPZ, United Kingdom²

Received 2 August 2000/Returned for modification 18 September 2000/Accepted 12 October 2000

Protective monoclonal antibodies (MAbs) to the major outer membrane protein (MOMP) of species of the family Chlamydiaceae, which is the primary vaccine candidate antigen, recognize nonlinear epitopes conferred by the oligomeric conformation of the molecule. Protective MAbs failed to recognize oligomeric MOMP of the variant strain LLG, which bears amino acid substitutions in variable segments (VSs) 1, 2, and 4, and competed with monomer-specific MAbs mapping to these VSs in reference strain 577. The results suggest that multiple sites located in the three VSs contribute to the epitope of protective MAbs.

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^* Corresponding author. Mailing address: Hellenic Pasteur Institute, 127 Vassilissis Sofias Ave., 115 21 Athens, Greece. Phone and fax: (301) 64 78 873. E-mail: evretou{at}netor.gr.

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Infection and Immunity, January 2001, p. 607-612, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.607-612.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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