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Infection and Immunity, January 2001, p. 613-616, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.613-616.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Differential Interleukin-8 Response of Intestinal Epithelial Cell Line to Reactogenic and Nonreactogenic Candidate Vaccine Strains of Vibrio cholerae

Boris L. Rodríguez,1,* Armando Rojas,2 Javier Campos,1 Talena Ledon,1 Edgar Valle,1 William Toledo,1 and Rafael Fando1

Departamento de Genética, Centro Nacional de Investigaciones Científicas,1 and Laboratorio de Farmacología, Centro de Química Farmacéutica,2 La Habana, Cuba

Received 18 April 2000/Returned for modification 16 June 2000/Accepted 25 October 2000

In this study, we analyzed whether attachment of Vibrio cholerae vaccine strains to human intestinal epithelial cells can induce an interleukin-8 (IL-8) response. The IL-8 transcripts were detected by PCR amplification of reverse-transcribed mRNA, and the gene product secretion was measured by an enzyme-linked immunosorbent assay. Infection of monolayers of the undifferentiated HT29-18N2 cell line with reactogenic (JBK70 and 81) and nonreactogenic (CVD103HgR and 638) vaccine strains of V. cholerae resulted in markedly higher IL-8 expression by epithelial cells exposed to reactogenic strains than by cells exposed to the nonreactogenic strains. Additionally, epithelial cells produced IL-8 transcripts following stimulation with cholera vaccine strains in a concentration-dependent manner. These results represent a new insight into the inflammatory component of reactogenicity and could be used as a predictive marker of vaccine reactogenicity prior to human testing.

* Corresponding author. Mailing address: Departamento de Genética, CNIC, Ave. 25 y 158, Cubanacan, Playa, P.O. Box 6412, La Habana, Cuba. Phone: (537) 21 80 66. Fax: (537) 28 04 97. E-mail: boris{at}biocnic.cneuro.edu.cu.

Infection and Immunity, January 2001, p. 613-616, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.613-616.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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