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 Previous Article

Infection and Immunity, January 2001, p. 622-625, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.622-625.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of the Domain of Fibronectin-Binding Protein I of Streptococcus pyogenes Responsible for Elicitation of a Protective Immune Response

Kai Schulze, Eva Medina, Susanne R. Talay, Rebecca J. Towers, Gursharan S. Chhatwal, and Carlos A. Guzmán*

Department of Microbial Pathogenesis and Vaccine Research, Division of Microbiology, GBF-German Research Centre for Biotechnology, D-38124 Braunschweig, Germany

Received 19 July 2000/Returned for modification 12 September 2000/Accepted 12 October 2000

Fibronectin-binding protein I (SfbI) represents a major adhesin of Streptococcus pyogenes. Mice were intranasally immunized with recombinant proteins spanning different portions of SfbI to identify the minimal fragment able to elicit a protective response against a lethal challenge with S. pyogenes. The strongest cellular responses and the highest levels of antigen-specific secretory immunoglobulin A (IgA) were detected in mice immunized with the fibronectin-binding region of SfbI. In contrast, animals vaccinated with a polypeptide spanning the aromatic and proline-rich regions showed the highest titers and fastest IgG response in serum. Vaccination with either SfbI without a membrane anchor and signal peptide or a polypeptide encompassing its fibronectin-binding regions resulted in efficient protection against heterologous challenge (60% and 80%, respectively), whereas the use of a polypeptide lacking this region conferred marginal protection (10%) with respect to the control group (0%). These results demonstrate that the fibronectin-binding region of SfbI is a promising candidate antigen for developing anti-S. pyogenes vaccines.

* Corresponding author. Mailing address: Vaccine Research Group, Department of Microbial Pathogenesis and Vaccine Research, Division of Microbiology, GBF-German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany. Phone: (49-531)6181558. Fax: (49-531)6181411. E-mail: cag{at}gbf.de.

Infection and Immunity, January 2001, p. 622-625, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.622-625.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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