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Infection and Immunity, January 2001, p. 75-80, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.75-80.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Inactivation of the srtA Gene in Streptococcus gordonii Inhibits Cell Wall Anchoring of Surface Proteins and Decreases In Vitro and In Vivo Adhesion

Tové C. Bolken,1 Christine A. Franke,1,2 Kevin F. Jones,1,2 Gloria O. Zeller,1 C. Hal Jones,1,2 Emma K. Dutton,1 and Dennis E. Hruby1,2,*

Siga Research Laboratories, Corvallis, Oregon 97333,1 and Department of Microbiology, Oregon State University, Corvallis, Oregon 973302

Received 23 August 2000/Returned for modification 20 September 2000/Accepted 27 September 2000

The srtA gene product, SrtA, has been shown to be required for cell wall anchoring of protein A as well as virulence in the pathogenic bacterium Staphylococcus aureus. There are five major mechanisms for displaying proteins at the surface of gram-positive bacteria (P. Cossart and R. Jonquieres, Proc. Natl. Acad. Sci. USA 97:5013-5015, 2000). However, since many of the known surface proteins of gram-positive bacteria are believed to be exported and anchored via the sortase pathway, it was of interest to determine if srtA plays a similar role in other gram-positive bacteria. To that end, the srtA gene in the human oral commensal organism Streptococcus gordonii was insertionally inactivated. The srtA mutant S. gordonii exhibited a marked reduction in quantity of a specific anchored surface protein. Furthermore, the srtA mutant had reduced binding to immobilized human fibronectin and had a decreased ability to colonize the oral mucosa of mice. Taken together, these results suggest that the activity of SrtA plays an important role in the biology of nonpathogenic as well as pathogenic gram-positive cocci.

* Corresponding author. Mailing address: SIGA Research Laboratories, 4575 S.W. Research Way, Suite 230, Corvallis, OR 97333. Phone: (541) 753-2000. Fax: (541) 753-9999. E-mail: dhruby{at}sgph.com.

Infection and Immunity, January 2001, p. 75-80, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.75-80.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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