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Infection and Immunity, October 2001, p. 6012-6021, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6012-6021.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Ferric Dicitrate Transport System (Fec) of Shigella flexneri 2a YSH6000 Is Encoded on a Novel Pathogenicity Island Carrying Multiple Antibiotic Resistance Genes

Shelley N. Luck,1 Sally A. Turner,1 Kumar Rajakumar,1,2 Harry Sakellaris,1,* and Ben Adler1

Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Victoria 3800,1 and Department of Microbiology and Infectious Diseases, Royal Children's Hospital, Parkville, Victoria 3052,2 Australia

Received 24 April 2001/Returned for modification 12 June 2001/Accepted 18 July 2001

Iron uptake systems which are critical for bacterial survival and which may play important roles in bacterial virulence are often carried on mobile elements, such as plasmids and pathogenicity islands (PAIs). In the present study, we identified and characterized a ferric dicitrate uptake system (Fec) in Shigella flexneri serotype 2a that is encoded by a novel PAI termed the Shigella resistance locus (SRL) PAI. The fec genes are transcribed in S. flexneri, and complementation of a fec deletion in Escherichia coli demonstrated that they are functional. However, insertional inactivation of fecI, leading to a loss in fec gene expression, did not impair the growth of the parent strain of S. flexneri in iron-limited culture media, suggesting that S. flexneri carries additional iron uptake systems capable of compensating for the loss of Fec-mediated iron uptake. DNA sequence analysis showed that the fec genes are linked to a cluster of multiple antibiotic resistance determinants, designated the SRL, on the chromosome of S. flexneri 2a. Both the SRL and fec loci are carried on the 66,257-bp SRL PAI, which has integrated into the serX tRNA gene and which carries at least 22 prophage-related open reading frames, including one for a P4-like integrase. This is the first example of a PAI that carries genes encoding antibiotic resistance and the first report of a ferric dicitrate uptake system in Shigella.

* Corresponding author. Mailing address: Department of Microbiology, Monash University, VIC 3800, Australia. Phone: 613 9905 4838. Fax: 613 9905 4811. E-mail: harry.sakellaris{at}med.monash.edu.au.

Infection and Immunity, October 2001, p. 6012-6021, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6012-6021.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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