Ferric Dicitrate Transport System (Fec) of Shigella flexneri 2a YSH6000 Is Encoded on a Novel Pathogenicity Island Carrying Multiple Antibiotic Resistance Genes

doi:10.1128/IAI.69.10.6012-6021.2001

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Infection and Immunity, October 2001, p. 6012-6021, Vol. 69, No. 10
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.10.6012-6021.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Ferric Dicitrate Transport System (Fec) of Shigella flexneri 2a YSH6000 Is Encoded on a Novel Pathogenicity Island Carrying Multiple Antibiotic Resistance Genes

Shelley N. Luck,¹ Sally A. Turner,¹ Kumar Rajakumar,¹^,² Harry Sakellaris,¹^,^* and Ben Adler¹

Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Victoria 3800,¹ and Department of Microbiology and Infectious Diseases, Royal Children's Hospital, Parkville, Victoria 3052,² Australia

Received 24 April 2001/Returned for modification 12 June 2001/Accepted 18 July 2001

Iron uptake systems which are critical for bacterial survival and which may play important roles in bacterial virulence areoften carried on mobile elements, such as plasmids and pathogenicityislands (PAIs). In the present study, we identified and characterizeda ferric dicitrate uptake system (Fec) in Shigella flexneri serotype2a that is encoded by a novel PAI termed the Shigella resistancelocus (SRL) PAI. The fec genes are transcribed in S. flexneri,and complementation of a fec deletion in Escherichia coli demonstratedthat they are functional. However, insertional inactivation offecI, leading to a loss in fec gene expression, did not impairthe growth of the parent strain of S. flexneri in iron-limitedculture media, suggesting that S. flexneri carries additionaliron uptake systems capable of compensating for the loss of Fec-mediatediron uptake. DNA sequence analysis showed that the fec genes arelinked to a cluster of multiple antibiotic resistance determinants,designated the SRL, on the chromosome of S. flexneri 2a. Boththe SRL and fec loci are carried on the 66,257-bp SRL PAI, whichhas integrated into the serX tRNA gene and which carries at least22 prophage-related open reading frames, including one for a P4-likeintegrase. This is the first example of a PAI that carries genesencoding antibiotic resistance and the first report of a ferricdicitrate uptake system in Shigella.

^* Corresponding author. Mailing address: Department of Microbiology, Monash University, VIC 3800, Australia. Phone: 613 99054838. Fax: 613 9905 4811. E-mail: harry.sakellaris{at}med.monash.edu.au.

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