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Infection and Immunity, October 2001, p. 6303-6309, Vol. 69, No. 10
Department of Microbiology, The University of
Sydney, Sydney, New South Wales 2006, Australia
Received 11 January 2001/Returned for modification 19 March
2001/Accepted 1 June 2001
Three genes, ipgD, mxiC, and mxiA,
all in the invasion region of the Shigella virulence
plasmid, were sequenced from strains representing a range of
Shigella serotypes and from two enteroinvasive Escherichia coli (EIEC) isolates. The plasmids can be
classified into two relatively homogeneous sequence forms which are
quite distinct. pINV A plasmids are found in Shigella
flexneri strains F6 and F6A, S. boydii strains
B1, B4, B9, B10, B14, and B15, S. dysenteriae strains
D3, D4, D6, D8, D9, D10, and D13, and the two EIEC strains (M519 and
M520). pINV B plasmids are present in S. flexneri
strains F1A, F2A, F3A, F3C, F4A, and FY, two S. boydii
strains (B11 and B12), and S. sonnei. The D1 pINV
plasmid is a recombinant with ipgD gene more closely
related to those of pINV A but with mxiA and
mxiC genes more closely related to those of pINV B. The
phylogenetic relationships of the plasmid and those of the chromosomal
genes of Shigella strains are largely consistent. The
cluster 1 and cluster 3 strains tested (G.M. Pupo, R. Lan, and P. R. Reeves, Proc. Natl. Acad. Sci. USA 97:10567-10572, 2000) have pINV
A and pINV B plasmids, respectively. However, of the three cluster 2 strains (B9, B11, and B15), B9 and B15 have pINV A while B11 has a pINV
B plasmid. Those Shigella (D8 and D10 and S.
sonnei) and EIEC strains which do not group with the main body
of Shigella strains based on chromosomal genes were found to have plasmids belonging to one or the other of the two types
and must have acquired these by lateral transfer.
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.10.6303-6309.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Molecular Evolution of Large Virulence Plasmid in
Shigella Clones and Enteroinvasive
Escherichia coli
*
Corresponding author. Mailing address: Department of
Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia. Phone: (612) 9351 2536. Fax: (612) 9351 4571. E-mail: reeves{at}angis.usyd.edu.au.
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