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Infection and Immunity, October 2001, p. 6511-6514, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6511-6514.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Epitope Mapping of Neutralizing Botulinum Neurotoxin A Antibodies by Phage Display

B. P. Mullaney,1,2,* M. G. Pallavicini,1,2,3 and J. D. Marks2,4

Departments of Laboratory Medicine,1 Radiation Oncology,3 and Anesthesia and Pharmaceutical Chemistry4 and Cancer Center,2 University of California at San Francisco, San Francisco, California 94143

Received 15 February 2001/Returned for modification 27 March 2001/Accepted 21 June 2001

Single-chain antibodies neutralize activity and bind nonoverlapping epitopes of botulinum A neurotoxin. Two phage display epitope libraries were constructed from the 1.3 kb of binding domain cDNA. The minimal epitopes selected against the single-chain Fv-Fc antibodies correspond to conformational epitopes with amino acid residues 1115 to 1223 (S25), 1131 to 1264 (3D12), and 889 to 1294 (C25).

* Corresponding author. Mailing address: UCSF Cancer Center, Box 0808, UCSF, San Francisco, CA 94143-0808. Phone: (415) 476-3657. Fax: (415) 476-8218. E-mail: mullaney{at}cc.ucsf.edu.

Infection and Immunity, October 2001, p. 6511-6514, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6511-6514.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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